While not specifically tested on gingival fibroblasts, this product is tested using mulitple mammalian cell lines. There is no reason to suspect that this product would not be suitable. For frozen cell stocks, the recommended maximum temperature is -70°C. Liquid nitrogen (-80°C) is a typical medium for this product. Please see the link below to review the product datasheet, which includes a brief protocol for freezing and thawing cells using this product:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/709/138/c6164pis.pdf
C6164
Medio de congelación celular-DMSO 1×
sterile-filtered, suitable for cell culture
Sinónimos:
cryopreservation medium
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
esterilidad
sterile-filtered
Nivel de calidad
Formulario
liquid
técnicas
cell culture | mammalian: suitable
cryopreservation: suitable
impurezas
endotoxin, tested
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
Descripción general
La congelación celular o la crioconservación de cultivos celulares es un proceso habitual. Es esencial para para mantener la integridad celular durante el almacenamiento. Los crioprotectores principalmente utilizados son el glicerol, el etilenglicol y el dimetilsulfóxido (DMSO).[1]
Aplicación
Otras notas
Medio completo listo para usar. Formulación patentada con medio esencial mínimo, sulfóxido de dimetilo, suero bovino y suero bovino fetal.
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves
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Artículos
Overview of good cell banking practices for cell line cryopreservation purposes.
Overview of good cell banking practices for cell line cryopreservation purposes.
Overview of good cell banking practices for cell line cryopreservation purposes.
Overview of good cell banking practices for cell line cryopreservation purposes.
Protocolos
Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.
Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.
Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.
Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.
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Good day will the cell- freezing medium - DMSO be suitable for freezing of human gingival fibroblsts? can they be stored in -80 freezer or rather in liquid nitrogen with this freezer medium?
1 answer-
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When is the expiry date after bottle has been opened?
1 answer-
Following opening, the stability of the product is contingent upon end user handling. Shelf life will be maintained unless contaminants are introduced or the product is improperly stored or sealed. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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How much DMSO is present in Product C6164, Cell Freezing Medium-DMSO?
1 answer-
The DMSO is at a 10% concentration.
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Is it ok to sterile filter Product C6164, Cell Freezing Medium-DMSO?
1 answer-
Product No. C6164 is sterile-filtered. If the sterility of the product has been compromised, it can be re-filtered.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Does Product C6164, Cell Freezing Medium-DMSO, contain heat-inactivated serum?
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No, the serum in this cell freezing media is not heat-inactivated.
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Is there insulin in Product C6164, Cell Freezing Medium-DMSO?
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No, there is no added insulin in this product. There may be traces of insulin from the serum used in this product.
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