This item is a product of PromoCell. The manufacturer considers the formulation proprietary. However, this is a complete medium that includes all the growth factors and supplements necessary for providing optimal cell health and efficient adherence selection of monocytes from freshly isolated mononuclear cells. This medium does not contain antibiotics or antimycotics and is formulated for use in an incubator with an atmosphere of 5% CO2.
C-28051
Monocyte Attachment Medium
250 ml
Sinónimos:
Primary Cell Culture Media
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.71
En este momento no podemos mostrarle ni los precios ni la disponibilidad
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form
aqueous solution
packaging
pkg of 250 ml
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
Monocyte Attachment Medium (Ready-to-use)
Application
The Monocyte Attachment Medium allows for the efficient adherence selection of monocytes from freshly isolated human mononuclear cells while maintaining optimal cell health. Thus, the time-consuming and costly immunomagnetic purification of monocytes is dispensable prior to experiments requiring adherent monocytes as a starting material. With properly performed washing steps large numbers of 80-90% pure monocytes can be obtained. Typical applications are generation of dendritic cells / macrophages as well as phagocytosis assays.Recommended for:Human mononuclear cells (freshly isolated)Human monocytes (freshly isolated)
Quality
All lots of PromoCell Monocyte Attachment Medium are subjected to comprehensive quality control tests using primary human mononuclear cells from peripheral blood. Each lot of Monocyte Attachment Medium is checked for the efficient adherence selection of highly viable monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Preparation Note
The Medium is chemically defined/xeno-free and does not need further supplementation.
Storage and Stability
Store the Medium at 2 to 8°C in the dark immediately after arrival. Do not freeze the Medium. If stored properly, the product is stable until the expiry date stated on the label. After opening, the shelf life of the medium is 6 weeks at 2 to 8°C. For use, pre-warm only an aliquot of the medium and keep the remaining medium refrigerated at 2 to 8°C.
Subculture Routine
Click here for more information.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.
Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.
Protocolos
Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.
Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.
Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.
Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.
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