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B7151

Sigma-Aldrich

Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

goat

Nivel de calidad

conjugado

biotin conjugate

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

secondary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

técnicas

direct ELISA: 1:200,000-1:300,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot: 1:400,000-1:800,000 using total cell extract of HeLa cells

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

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Descripción general

IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. IgG is found in four subclasses IgG1, IgG2, IgG3 and IgG4. IgG has a monomeric structure.
The product binds to all mouse Igs

Aplicación

Anti-Mouse IgG (Fab specific)-Biotin antibody produced in goat was used in immunohistochemistry of lateral and medial menisci of rabbits and human adipose-derived stem cells.

Acciones bioquímicas o fisiológicas

IgG (immunoglobulin G) antibody participates in complement fixation and opsonization.
IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to Anti-Mouse IgG (Fab specific) antibody allows for the binding of various labels such as avidin or streptavidin.

Otras notas

Antibody adsorbed with bovine, equine and human serum proteins.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Nota de preparación

Adsorbed to reduce background staining with bovine, horse or human samples.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Joseph F Poduslo et al.
Journal of neurochemistry, 102(2), 420-433 (2007-06-29)
Targeting therapeutic or diagnostic proteins to the nervous system is limited by the presence of the blood-brain barrier. We report that a F(ab')(2) fragment of a monoclonal antibody against fibrillar human Abeta42 that is polyamine (p)-modified has increased permeability at
Brian O Diekman et al.
Journal of biomedical materials research. Part A, 93(3), 994-1003 (2009-09-02)
Adipose-derived stem cells (ASCs) are multipotent progenitors that can be chondrogenically induced by growth factors such as bone morphogenetic protein 6 (BMP-6). We hypothesized that nonviral transfection of a BMP-6 construct (pcDNA3-BMP6) would induce chondrogenic differentiation of ASCs encapsulated in
Franklin T Moutos et al.
Tissue engineering. Part A, 16(4), 1291-1301 (2009-11-12)
Articular cartilage possesses complex mechanical properties that provide healthy joints the ability to bear repeated loads and maintain smooth articulating surfaces over an entire lifetime. In this study, we utilized a fiber-reinforced composite scaffold designed to mimic the anisotropic, nonlinear
G M Cunniffe et al.
European cells & materials, 33, 130-142 (2017-02-16)
Limitations associated with demineralised bone matrix and other grafting materials have motivated the development of alternative strategies to enhance the repair of large bone defects. The growth plate (GP) of developing limbs contain a plethora of growth factors and matrix
Franklin T Moutos et al.
Macromolecular bioscience, 10(11), 1355-1364 (2010-09-22)
The successful replacement of large-scale cartilage defects or osteoarthritic lesions using tissue-engineering approaches will likely require composite biomaterial scaffolds that have biomimetic mechanical properties and can provide cell-instructive cues to control the growth and differentiation of embedded stem or progenitor

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