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Merck

A8582

Sigma-Aldrich

Antifoam SE-15

aqueous emulsion for bacterial and mammalian systems

Sinónimos:

silicone-based antifoam

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About This Item

UNSPSC Code:
12161901
NACRES:
NA.85

Quality Level

form

emulsion (aqueous)

concentration

10% (active silicon)

technique(s)

cell culture | hybridoma: suitable

solubility

H2O: soluble (effective hot or cold.)

density

1.0 g/mL at 25 °C

application(s)

microbiology

storage temp.

room temp

suitability

(Mammalian (suspension))
bacteria (fermentation)

General description

Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Application

Antifoam SE-15 has been used:

  • in the preparation of ramified rolling circle amplification (RAM) amplification buffer.
  • to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.
  • as a constituent of phosphate-buffered saline (PBS) for the determination of total antioxidant status (TAOS) by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay.
  • as a component of autoinduction expression culture.
  • as a component of cyclic adenosine monophosphate (cAMP) detection buffer.

Features and Benefits

  • Controls foaming in microbial culture flasks or fermentations
  • Non-toxic to the culture medium
  • Can be pumped to fermenter on an as-needed basis

Components

Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .

Preparation Note

Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted
suspensions should be used immediately.

related product

Referencia del producto
Descripción
Precios

pictograms

Health hazardExclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - STOT RE 2 Inhalation

target_organs

Lungs

wgk_germany

WGK 1


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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