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Merck

A8011

Sigma-Aldrich

Antifoam C Emulsion

aqueous-silicone emulsion

Sinónimos:

Antifoaming Agent Emulsion

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About This Item

UNSPSC Code:
12161900
NACRES:
NA.25

biological source

synthetic

form

emulsion

contains

emulsifier

technique(s)

cell culture | hybridoma: suitable
microbiological culture: suitable

mp

0  °C (32 °F)

General description

Antifoam C emulsion is an aqueous emulsion containing 30% active silicone. This silicone-based antifoam emulsion consists of non-ionic emulsifiers different from those in antifoam emulsions B and Y-30. Antifoam C emulsion can be prediluted with 3-10 parts of cool water to aid in dispersion. Prediluted suspensions should be used immediately. Antifoam C emulsion is typically effective at 1-10 ppm. The flow properties of antifoam C emulsion are such that it can be pumped on an as-needed basis to a fermenter system with sufficient agitation to disperse the antifoam. Antifoam C emulsion can be used to regulate headspace foaming in mammalian cell culture bioreactors up to 30 ppm concentration.

Application

Antifoam C emulsion has been used:
  • as a supplement in RO-H2O and a chemically defined culture medium to determine volumetric mass transfer coefficient (kLa) of mammalian cell culture
  • as a component of feed medium,
  • as a steam-sterilized antifoaming agent in a biocontroller for pH-stat fed-batch cultivations
  • as a supplement in fed batch medium to mimic the typical large-scale cultivation conditions

Features and Benefits

  • Minimizes compatibility issues with biological systems
  • Highly effective defoamer at low concentrations
  • Highly effective and versatile silicone defoamer

Other Notes

A 30% aqueous emulsion of polydimethylsiloxane concentrate.
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pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


Certificados de análisis (COA)

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