A6805

β-N-Acetylglucosaminidase from Streptococcus pneumoniae

recombinant, expressed in E. coli, buffered aqueous solution

• Sinónimos: β-N-Acetyl-D-hexosaminide N-acetylhexosaminohydrolase, β-N-Acetylhexosaminidase
• Número de CAS: 9012-33-3
• Comisión internacional de enzimas: 3.2.1.52 (BRENDA, IUBMB)
• Número CE: 232-730-2
• Número MDL: MFCD00081402
• Código UNSPSC: 12352204
• NACRES: NA.32

Propiedades

• recombinante: expressed in E. coli
• Nivel de calidad: 100
• Formulario: buffered aqueous solution
• actividad específica: ≥80 units/mg protein
• envase: vial of ≥1.0 unit
• actividad extraña: β-galactosidase, α-mannosidase, α-fucosidase, neuraminidase, and proteases, none detected (Enzyme is expressed in glycosidase-free host.)
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: Streptococcus pneumoniae R6 ... lytB(934406)

Descripción

Aplicación

β-N-Acetylglucosaminidase from Streptococcus pneumoniae has been used in the removal of O-linked sugars from inositol 1,4,5-trisphosphate (InsP3) receptor type I.[1] It has also been used in coating PVDF strips for amelogenin trityrosyl motif peptide (ATMP) and keratin 5 binding studies.[2]

Acciones bioquímicas o fisiológicas

β-N-Acetylglucosaminidase from Streptococcus pneumoniae is an extracellular glycosidase and has broad specificity over the β1-3 and β1-6 linkages present in mucins.[3] It belongs to the glycoside hydrolase family 85 (GH85).[4] Also called endo-β-N-acetylglucosaminidase, it cleaves at the asparagine residue of the di-N-acetylchitobiose structure in oligosaccharides[5] and acts on fucosylated N-glycan core.[4] Its deglycosylation functionality is exploited in studying functions of glycoproteins.[5]

Specific for GlcNAc; not significantly active with GalNAc

This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.

Definición de unidad

One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at the pH 5 at 37 °C.

Forma física

Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable