Yes, 10% of the culture volume is suitable for use. The kit instructions require 10uL of reagent per 100ul culture volume in a 96 well format. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/381/017/96992dat.pdf
Please see the following link to review troubleshooting tips that may also be of interest:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/129/020/96992-product-information.pdf
96992
Kit para recuento celular - 8
for quantitation of viable cell number in proliferation and cytotoxicity assays
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| Talla/SKU | Disponibilidad | Precio |
|---|
Acerca de este artículo
NACRES:
NA.32
UNSPSC Code:
12352108
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Servicio técnico
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Permítanos ayudarleQuality Segment
form
liquid
shelf life
limited shelf life, expiry date on the label
color
lightdeep red
pH
6-8
solubility
water: soluble
shipped in
ambient
storage temp.
2-8°C
Application
El kit de recuento celular-8 (CCK-8) permite análisis cómodos utilizando WST-8 (2-(2-metoxi-4-nitrofenil)-3-(4-nitrofenil)-5-(2,4-disulfofenil)-2H-tetrazolio, sal monosódica), que produce un colorante formazán hidrosoluble mediante biorreducción en presencia de un transportador de electrones, el 1-metoxi PMS. La disolución CCK-8 se añade directamente a las células; no se necesita premezcla de los componentes. El WST-8 es biorreducido por las deshidrogenasas celulares a un producto formazán naranja que es soluble en el medio de cultivo tisular. La cantidad de formazán producida es directamente proporcional al número de células vivas. Dado que la disolución CCK-8 es muy estable y que tiene poca citotoxicidad, es posible una incubación más prolongada, del tipo de 24 a 48 horas.
El kit de recuento celular-8 (Cell Counting Kit-8) permite valoraciones colorimétricas sensibles para la determinación del número de células viables en los análisis de proliferación y citotoxicidad. La sensibilidad de detección es mayor que la de cualquier otra sal de tetrazolio, como MTT, XTT o MTS.
El kit de recuento celular-8 (Cell Counting Kit-8) permite valoraciones colorimétricas sensibles para la determinación del número de células viables en los análisis de proliferación y citotoxicidad. La sensibilidad de detección es mayor que la de cualquier otra sal de tetrazolio, como MTT, XTT o MTS.
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Este artículo | |||
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| description for quantitation of viable cell number in proliferation and cytotoxicity assays | description Animal-protein free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | description protein free, liquid, sterile-filtered, suitable for cell culture | description Chemically defined, powder, suitable (for insect cell culture) |
| color lightdeep red | color - | color - | color - |
| pH 6-8 | pH - | pH - | pH - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility water: soluble | solubility - | solubility - | solubility - |
| shelf life limited shelf life, expiry date on the label | shelf life - | shelf life - | shelf life - |
| form liquid | form liquid | form liquid | form dry powder |
Palabra de advertencia
Warning
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2
Clase de almacenamiento
12 - Non Combustible Liquids
¿Qué está pasando?
WGK 2
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
EPI (equipos de protección individual)
Eyeshields, Gloves
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How, much volume to be used for 12 well plate. For e.g I am growing cells in my 12 well plate over a thin film. some paper mentions to use 10%, so if I add 100ul with 1ml media. will it work?
1 answer-
Helpful?
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What is the solution volume provided in the "3000 tests"?
1 answer-
The 96992-3000TESTS vial will include 30 milliliters of reagent.
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100 Test means 100 wells, so only one 96-well plate?
1 answer-
The reagents provided in kit 96992 are sufficient for 100 tests. A 96 well microplate is not included with the kit. The customer must provide the 96 well plate. Although a solid 96 well plate can be used, a 96 well microplate with removable strips will allow for the most efficient use of the kit.
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How much volume (in milliliters) is present in each vial of the CCK-8 solution?
1 answer-
The volume supplied for Product No. 96992, Cell Counting Kit, is as follows:
•100 tests: 1 ml x 1 vial
•500 tests: 5 ml x 1 vial
•3000 tests: 5 ml x 6 vialsHelpful?
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What are the volumes of reagents included in the 96992-100test-F kit?
1 answer-
Supplier information specifies that the kit contains 6 bottles, each containing 5 ml of solution. Based on the product datasheet, each well of a well-plate requires 10 microliters of solution, indicating that for 3000 tests, the kit should contain 30 ml of solution. Using these calculations, for the 100 test kit, each bottle would contain approximately 167 microliters of solution (5 ml divided by 30 = 167 microliters).
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Is this kit suitable for use with HEK293 or HEK293f cells?
1 answer-
Yes, the 96992 kit can be utilized to stain both HEK293 and HEK293B cells. The Cell Count kit is not cell type-specific and is expected to function effectively for counting viable mammalian cells. In this kit, the WST-8 dye, which is a formazan stain is specific to viable cells and is not present in nonviable cells.
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Hi, what is the solution volume provided in the "500 tests" SKU? Thanks in advance, Aviv.
1 answer-
The 500 test volume is 5 mL. See the links below to the Product datasheet and the Product Troubleshooting Guide for additional information.
Product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/381/017/96992dat.pdfTroubleshooting Guide:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/129/020/96992-product-information.pdfHelpful?
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