56436C

Medio de Eagle modificado de Dulbecco, glucosa elevada

with 4500 mg/L dextrose, with 4.0 mM L-glutamine, without phenol red, without sodium bicarbonate, without sodium pyruvate, powder, suitable for cell culture

• Sinónimos: DME, DMEM
• UNSPSC Code: 12352200
• NACRES: NA.71

Propiedades

• description: for research or for further manufacturing use
• Quality Level: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: phenol red: no; L-glutamine: yes; glucose: high; sodium pyruvate: no
• shipped in: ambient
• storage temp.: 2-8°C

Descripción

Application

El medio de Eagle modificado de Dulbecco (DMEM) es una modificación del medio basal Eagle (BME) que contiene el cuádruple de concentraciones de aminoácidos y vitaminas. La formulación original contenía 1000 mg/l de glucosa y se utilizaba para cultivar células embrionarias de ratón. Desde entonces, se ha modificado de varias formas para soportar cultivos primarios de células de ratón y de pollo, así como una variedad de células normales y transformadas. Cada uno de estos medios ofrece una combinación diferente de L-glutamina y piruvato sódico. Además, los niveles de glucosa se han elevado a 4 500 mg/l, lo que contribuye al nombre «DMEM/High».

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of dextrose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Additionally, the dextrose level has been raised to 4500 mg/L, contributing to the name "DMEM/High."

Quantity

Formulated to contain 13.4 grams of powder per liter of medium.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable