It is possible that either too much glacial acetic acid was added, or the acetic acid was added too quickly. When using the 0.5% Eosin Y solution sold as catalog number HT110216-500 ml, the instructions recommend adding 0.5 glacial acetic acid slowly to 100 ml of stain solution just prior to use. It's not uncommon to observe this in the lab. In most cases, the precipitate can be resolved by swirling the solution by hand or mixing it on a stir plate.
318906
Eosin Y solution
5 wt. % in H2O
Sinónimos:
2′,4′,5′,7′-Tetrabromofluorescein disodium salt, Acid red 87, Eosin yellowish solution
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About This Item
Fórmula empírica (notación de Hill):
C20H6Br4Na2O5
Número de CAS:
Peso molecular:
691.85
Beilstein/REAXYS Number:
1443944
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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form
liquid
Quality Level
concentration
5 wt. % in H2O
color
red to very dark red
density
1.015 g/mL at 25 °C
λmax
518 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
InChI
1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
InChI key
SEACYXSIPDVVMV-UHFFFAOYSA-L
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General description
Eosin Y, also known as Eosin yellowish is a widely used xanthene dye. It is a counterstain that is used to distinguish between various connective tissue fibers & matrices, and the cytoplasm of different types of cells. Eosin in combination with hematoxylin is useful to demonstrate the general histological architecture of a tissue.
Application
Eosin Y is suitable for use in hematoxylin and eosin (H&E) staining procedures for various tissue samples like lung nodule and kidney sections.
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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a 1% Eosin Y solution in water using the product under the catalog number 318906 (Eosin Y solution 5wt% in water) was created and then diluted to 1% in water, the pH was lowered with acetic acid, but it immediately changed to a bright red color and produced a precipitate?
1 answer-
Helpful?
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Is Product 318906 suitable for use in Hematoxylin and Eosin (H&E) staining procedures?
1 answer-
Product 318906 was not intended for use with H&E staining techniques. The dye content for the Eosin Y is 5% in water. For H&E staining, the concentration is typically 0.5 to 1.0 %. At 5 % dye content, the dye is much too concentrated for use with H&E staining. If used at such a high concentration, the cytoplasmic staining is likely to obscure the nuclear staining from the hematoxylin. A well stained H&E slide should show blue nuclear staining and typically at least 3 shades of Eosin staining. When used at 5% dye content, the cytoplasmic staining is likely to be a homogenous or monotone.The 5% Eosin solution has only one common application - determining sperm viability. In a typical semen analysis, 5% Eosin Y is used in conjunction with 10% Nigrosin. This combination of dyes is used to judge the viability of the sperm. A standard H&E procedure is then used to judge the morphology of the sperm. Sperm counts are typically performed without the use of a dye solution.It is possible to consider a 5% Eosin solution as a 10X staining solution. The product, however, is not suitability tested for use in either a sperm viability procedure or a H&E staining procedure.The following products have been suitability tested for use in H&E staining procedures and are considered suitable for use in In Vitro Diagnostic procedures. They are the preferred products for H&E staining. Most are formulated with 0.5% Eosin Y. The exception is Eosin Y with Phloxine B. For this formulation both the Eosin Y and the Phloxine B are formulated at 0.1% dye content.Eosin Y solution, alcoholicHT110116, HT110132, HT110180, HT1101128Eosin Y solution, aqueousHT110216, HT110232, HT110280, HT1102128Eosin Y solution, alcoholic, with phloxineHT110316, HT110332, HT110380, HT1103128
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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