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70584-M

Millipore

Reactivo para extracción de proteínas BugBuster®

Sinónimos:

Disolución de extracción de proteínas, Reactivo de extracción de proteínas

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About This Item

Código UNSPSC:
41106516
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Formulario

liquid

Nivel de calidad

100

fabricante / nombre comercial

Novagen®

condiciones de almacenamiento

OK to freeze
avoid repeated freeze/thaw cycles

temp. de almacenamiento

10-30°C

Descripción general

El reactivo de extracción de proteínas BugBuster® está formulado para la ruptura suave de la pared celular de Escherichia coli para liberar proteínas activas. Proporciona una alternativa sencilla, rápida y de bajo coste a métodos mecánicos como la prensa francesa o la administración de ultrasonidos para liberar la proteína diana expresada en preparación para la purificación u otras aplicaciones. La formulación patentada utiliza una mezcla basada en tampón Tris de detergentes no iónicos y dipolares que es capaz de perforar la pared celular sin desnaturalizar la proteína. BugBuster también es útil para la preparación de cuerpos de inclusión de gran pureza para posterior purificación en casos en los que las proteínas expresadas son insolubles.

Aplicación

El reactivo de extracción de proteínas BugBuster® se ha utilizado:
  • para lisar las células BLR (DE3) de Escherichia coli para la producción y la purificación de β-glucosidasa (BgIB)[1]
  • como tampón de lisis celular para tratar los transconjugantes de la cepa PCC6803 de Synechocystis y medir la fluorescencia de la proteína fluorescente amarilla (YFP)[2]
  • en tampón de lisis para la purificación de la glutatión S-transferasa -proteína de choque térmico A (Hsp70) miembro 8 (GST- HSPA8)[3]

Advertencia

Toxicidad: Manipulación estándar (A)

Información legal

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Patricia Armshaw et al.
Biotechnology for biofuels, 8, 201-201 (2015-12-03)
The use of photosynthetic autotrophs and in particular the model organism Synechocystis PCC6803 is receiving much attention for the production of sustainable biofuels and other economically useful products through metabolic engineering. Optimisation of metabolic-engineered organisms for high-level sustained production of
Dylan Alexander Carlin et al.
PloS one, 12(5), e0176255-e0176255 (2017-05-23)
Accurate modeling of enzyme activity and stability is an important goal of the protein engineering community. However, studies seeking to evaluate current progress are limited by small data sets of quantitative kinetic constants and thermal stability measurements. Here, we report
Tiejian Nie et al.
Autophagy, 17(10), 2923-2938 (2021-05-11)
As a highly dynamic organelle, mitochondria undergo constant fission and fusion to change their morphology and function, coping with various stress conditions. Loss of the balance between fission and fusion leads to impaired mitochondria function, which plays a critical role

Artículos

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

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Contenido relacionado

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

Lisis y extracción de proteínas

Los métodos de lisis celular y extracción de proteínas abarcan varias técnicas, desde la solubilización de detergentes hasta la ruptura mecánica, que respaldan las necesidades de investigación.

Ver todo

Questions

1–3 of 3 Questions  

  1. Can this be used to extract membrane proteins?

    1 answer

    1. BugBuster Protein Extraction Reagent is a mixture of non-ionic and zwitterionic detergents optimized for the extraction of soluble proteins. It does not solubilize insoluble proteins in inclusion bodies. BugBuster does not lyse cells but creates openings in the membrane to release proteins, similar to most detergent-based extractions. Traditionally, detergents are used to release membrane proteins into the soluble fraction, although not all membrane proteins will be released. Some may remain in the pellet and require denaturing conditions for release. Highly hydrophobic or membrane-associating proteins are likely to be found in the cell debris pellet. The extraction of membrane proteins using BugBuster has not been directly evaluated. Non-ionic detergents can solubilize some membrane proteins, but the extent to which BugBuster achieves this has not been analyzed. It is expected that some, but not all, membrane proteins would be solubilized using BugBuster, and therefore, its use for membrane protein extraction cannot be guaranteed.

      Helpful?

  2. can we use this for protein extarction of P aeruginosa bacteria for Carb NP test

    1 answer

    1. BugBuster® Protein Extraction Reagent is formulated for gentle disruption of E. coli cell wall. It is not tested for use with other organisms. Suitability would have to be determined experimentally.

      Helpful?

  3. Can I use bugbuster to lysis Mycobacterium tuberculosis(ATCC25177)?

    1 answer

    1. BugBuster® Protein Extraction Reagent is formulated for gentle disruption of E. coli cell wall. It is not tested for use with other organisms. Suitability would have to be determined experimentally.

      Helpful?

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