BugBuster Protein Extraction Reagent is a mixture of non-ionic and zwitterionic detergents optimized for the extraction of soluble proteins. It does not solubilize insoluble proteins in inclusion bodies. BugBuster does not lyse cells but creates openings in the membrane to release proteins, similar to most detergent-based extractions. Traditionally, detergents are used to release membrane proteins into the soluble fraction, although not all membrane proteins will be released. Some may remain in the pellet and require denaturing conditions for release. Highly hydrophobic or membrane-associating proteins are likely to be found in the cell debris pellet. The extraction of membrane proteins using BugBuster has not been directly evaluated. Non-ionic detergents can solubilize some membrane proteins, but the extent to which BugBuster achieves this has not been analyzed. It is expected that some, but not all, membrane proteins would be solubilized using BugBuster, and therefore, its use for membrane protein extraction cannot be guaranteed.
70584-M
Reactivo para extracción de proteínas BugBuster®
Sinónimos:
Disolución de extracción de proteínas, Reactivo de extracción de proteínas
About This Item
Productos recomendados
form
liquid
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
storage temp.
10-30°C
General description
Application
- para lisar las células BLR (DE3) de Escherichia coli para la producción y la purificación de β-glucosidasa (BgIB)[1]
- como tampón de lisis celular para tratar los transconjugantes de la cepa PCC6803 de Synechocystis y medir la fluorescencia de la proteína fluorescente amarilla (YFP)[2]
- en tampón de lisis para la purificación de la glutatión S-transferasa -proteína de choque térmico A (Hsp70) miembro 8 (GST- HSPA8)[3]
Warning
Legal Information
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Artículos
Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens
This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.
This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.
This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.
Contenido relacionado
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Los métodos de lisis celular y extracción de proteínas abarcan varias técnicas, desde la solubilización de detergentes hasta la ruptura mecánica, que respaldan las necesidades de investigación.
-
Can this be used to extract membrane proteins?
1 answer-
Helpful?
-
-
can we use this for protein extarction of P aeruginosa bacteria for Carb NP test
1 answer-
BugBuster® Protein Extraction Reagent is formulated for gentle disruption of E. coli cell wall. It is not tested for use with other organisms. Suitability would have to be determined experimentally.
Helpful?
-
-
Can I use bugbuster to lysis Mycobacterium tuberculosis(ATCC25177)?
1 answer-
BugBuster® Protein Extraction Reagent is formulated for gentle disruption of E. coli cell wall. It is not tested for use with other organisms. Suitability would have to be determined experimentally.
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico