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Supelco

Chromolith® WP 300 Protein A (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

Sinónimos:

Chromolith® WP 300 Protein A 25-4.6 HPLC column

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About This Item

Código UNSPSC:
41115700
NACRES:
SB.52

Nombre del producto

Chromolith® WP 300 Protein A, L × I.D. 25 mm x 4.6 mm HPLC column

Materiales

PEEK column

Línea del producto

Chromolith®

Características

endcapped: no
endcapped: no

envase

1 ea of

Parámetros

2 - 45 °C temp. range
2-45 °C temp. range
200 bar pressure
200 bar pressure

técnicas

HPLC: suitable
HPLC: suitable
LC/MS: suitable

columna L × D.I.

2.5 cm × 4.6 mm

superficie

120 m2/g

Matriz

monolithic silica
monolithic silica

grupo activo de la matriz

Protein A bonding phase
Protein A bonding phase

tamaño de poro

2 μm macroporosity
2 μm macroporosity
30 nm mesoporosity
30 nm mesoporosity

Intervalo de pH operativo

1.5-7.5

pH operativo

1.5-7.5

técnica de separación

affinity

temp. de almacenamiento

2-8°C
2-8°C

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Descripción general

Affinity chromatography is a selective technique which takes advantage of specific molecular interactions, for example antigen and antibody. The Chromolith® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.

Chromolith® WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Chromolith® WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Información legal

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

Contenido relacionado

La HPLC separa e identifica biomoléculas grandes como proteínas y péptidos mediante el bombeo de la muestra a través de una columna rellena de adsorbente.

La HPLC separa e identifica biomoléculas grandes como proteínas y péptidos mediante el bombeo de la muestra a través de una columna rellena de adsorbente.

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

Ver todo

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