- Complete sequencing and characterization of 21,243 full-length human cDNAs.
Complete sequencing and characterization of 21,243 full-length human cDNAs.
Nature genetics (2004-01-01)
Toshio Ota, Yutaka Suzuki, Tetsuo Nishikawa, Tetsuji Otsuki, Tomoyasu Sugiyama, Ryotaro Irie, Ai Wakamatsu, Koji Hayashi, Hiroyuki Sato, Keiichi Nagai, Kouichi Kimura, Hiroshi Makita, Mitsuo Sekine, Masaya Obayashi, Tatsunari Nishi, Toshikazu Shibahara, Toshihiro Tanaka, Shizuko Ishii, Jun-ichi Yamamoto, Kaoru Saito, Yuri Kawai, Yuko Isono, Yoshitaka Nakamura, Kenji Nagahari, Katsuhiko Murakami, Tomohiro Yasuda, Takao Iwayanagi, Masako Wagatsuma, Akiko Shiratori, Hiroaki Sudo, Takehiko Hosoiri, Yoshiko Kaku, Hiroyo Kodaira, Hiroshi Kondo, Masanori Sugawara, Makiko Takahashi, Katsuhiro Kanda, Takahide Yokoi, Takako Furuya, Emiko Kikkawa, Yuhi Omura, Kumi Abe, Kumiko Kamihara, Naoko Katsuta, Kazuomi Sato, Machiko Tanikawa, Makoto Yamazaki, Ken Ninomiya, Tadashi Ishibashi, Hiromichi Yamashita, Katsuji Murakawa, Kiyoshi Fujimori, Hiroyuki Tanai, Manabu Kimata, Motoji Watanabe, Susumu Hiraoka, Yoshiyuki Chiba, Shinichi Ishida, Yukio Ono, Sumiyo Takiguchi, Susumu Watanabe, Makoto Yosida, Tomoko Hotuta, Junko Kusano, Keiichi Kanehori, Asako Takahashi-Fujii, Hiroto Hara, Tomo-o Tanase, Yoshiko Nomura, Sakae Togiya, Fukuyo Komai, Reiko Hara, Kazuha Takeuchi, Miho Arita, Nobuyuki Imose, Kaoru Musashino, Hisatsugu Yuuki, Atsushi Oshima, Naokazu Sasaki, Satoshi Aotsuka, Yoko Yoshikawa, Hiroshi Matsunawa, Tatsuo Ichihara, Namiko Shiohata, Sanae Sano, Shogo Moriya, Hiroko Momiyama, Noriko Satoh, Sachiko Takami, Yuko Terashima, Osamu Suzuki, Satoshi Nakagawa, Akihiro Senoh, Hiroshi Mizoguchi, Yoshihiro Goto, Fumio Shimizu, Hirokazu Wakebe, Haretsugu Hishigaki, Takeshi Watanabe, Akio Sugiyama
PMID14702039
RESUMEN
As a base for human transcriptome and functional genomics, we created the "full-length long Japan" (FLJ) collection of sequenced human cDNAs. We determined the entire sequence of 21,243 selected clones and found that 14,490 cDNAs (10,897 clusters) were unique to the FLJ collection. About half of them (5,416) seemed to be protein-coding. Of those, 1,999 clusters had not been predicted by computational methods. The distribution of GC content of nonpredicted cDNAs had a peak at approximately 58% compared with a peak at approximately 42%for predicted cDNAs. Thus, there seems to be a slight bias against GC-rich transcripts in current gene prediction procedures. The rest of the cDNAs unique to the FLJ collection (5,481) contained no obvious open reading frames (ORFs) and thus are candidate noncoding RNAs. About one-fourth of them (1,378) showed a clear pattern of splicing. The distribution of GC content of noncoding cDNAs was narrow and had a peak at approximately 42%, relatively low compared with that of protein-coding cDNAs.
MATERIALES
Referencia del producto
Marca
Descripción del producto
Sigma-Aldrich
Lipase from porcine pancreas, Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Sigma-Aldrich
Acetilcolinesterasa from Electrophorus electricus (electric eel), Type VI-S, lyophilized powder, 200-1,000 units/mg protein
Sigma-Aldrich
Alcohol Dehydrogenase from Saccharomyces cerevisiae, ≥300 units/mg protein, lyophilized powder (contains buffer salts), Mw 141-151 kDa
Sigma-Aldrich
L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
Sigma-Aldrich
Creatine Phosphokinase from rabbit muscle, Type I, salt-free, lyophilized powder, ≥150 units/mg protein
Sigma-Aldrich
ADN polimerasa Taq from Thermus aquaticus, with 10× PCR reaction buffer without MgCl2
Sigma-Aldrich
ADN polimerasa Taq from Thermus aquaticus, with 10× PCR reaction buffer containing MgCl2
Sigma-Aldrich
β-Galactosidasa from Escherichia coli, Grade VIII, lyophilized powder, ≥500 units/mg protein
Sigma-Aldrich
Alcohol Dehydrogenase from Saccharomyces cerevisiae, powder, ≥300 units/mg protein, mol wt ~141,000 (four subunits)
Sigma-Aldrich
Lipase from porcine pancreas, Type VI-S, ≥20,000 units/mg protein, lyophilized powder
Sigma-Aldrich
Pyruvate Kinase from rabbit muscle, Type III, lyophilized powder, 350-600 units/mg protein
Sigma-Aldrich
Diaforasa from Clostridium kluyveri, lyophilized powder, 3.0-20.0 units/mg protein (biuret)
Sigma-Aldrich
Hexokinase from Saccharomyces cerevisiae, Type F-300, lyophilized powder, ≥130 units/mg protein (biuret)
Sigma-Aldrich
Lipase B Candida antarctica, recombinant from Aspergillus oryzae, powder, beige, ~9 U/mg
Sigma-Aldrich
Monoamine Oxidase B human, recombinant, expressed in baculovirus infected BTI insect cells
Sigma-Aldrich
L-Lactic Dehydrogenase from rabbit muscle, Type II, ammonium sulfate suspension, 800-1,200 units/mg protein
Sigma-Aldrich
Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle, lyophilized powder, ≥75 units/mg protein
Sigma-Aldrich
Acetilcolinesterasa from Electrophorus electricus (electric eel), Type V-S, lyophilized powder, ≥1,000 units/mg protein
Sigma-Aldrich
L-Lactic Dehydrogenase from bovine heart, Type III, ammonium sulfate suspension, ≥500 units/mg protein
Sigma-Aldrich
Pyruvate Kinase from rabbit muscle, Type II, ammonium sulfate suspension, 350-600 units/mg protein
Sigma-Aldrich
Asparaginase from Escherichia coli, lyophilized powder, 100-300 units/mg protein (biuret)
Sigma-Aldrich
Glutamic-Oxalacetic Transaminase from porcine heart, Type I, ammonium sulfate suspension, 200-500 units/mg protein
Sigma-Aldrich
Monoamine Oxidase A human, recombinant, expressed in baculovirus infected BTI insect cells