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P3627

Sigma-Aldrich

Phosphatase, Acid from wheat germ

≥0.4 unit/mg solid

Sinónimos:

(Orthophosphoric-monoester phosphohydrolase [acid optimum] ), Acid Phosphatase from wheat germ

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About This Item

Número de CAS:
9001-77-8
Comisión internacional de enzimas:
3.1.3.2 (BRENDA, IUBMB)
EC Number:
232-630-9
MDL number:
MFCD00131847
UNSPSC Code:
12352204
eCl@ss:
42010102
NACRES:
NA.54

form

powder

specific activity

≥0.4 unit/mg solid

mol wt

58 kDa by gel filtration

storage temp.

−20°C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI key

GZCWLCBFPRFLKL-UHFFFAOYSA-N

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Application

Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Product P3627 is from wheat germ and has been used to determine the effect of phosphatase treatment on 3F3/2 staining.

Biochem/physiol Actions

Plant acid phosphatase (APase) is localized in cytosol, vacuoles, and cell walls. Plant acid phosphatase is important for phosphate acquisition to mobilize organic phosphates in the soil. Phosphate starvation induces acid phosphatase generation. Acid phosphatase originates from the aleurone and scutellar tissues during germination. It hydrolyzes phytins, ATP, protein phosphates and nucleotide phosphates. It has a role in general metabolic reactions. Wheat embryo APase consists of four isoenzymes. The molecular mass of wheat germ APase is 58 kDa (gel filtration), the pH Optimum is 5.7, the pH Range is 4.0–7.0 and the optimal temperature is 45 °C.

Quality

Contains lipase

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Preparation Note

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Zuzana Novotná et al.
FEBS letters, 554(1-2), 50-54 (2003-11-05)
Phospholipase D (PLD) forms the major family of phospholipases that was first discovered and cloned in plants. In this report we have shown, for the first time, that C2 phosphatidylinositol-4,5-bisphosphate (PIP2)-dependent PLD(s) from 5 day hypocotyls of Brassica oleracea associated
H G Yu et al.
The Journal of cell biology, 145(3), 425-435 (1999-05-04)
We have identified a maize homologue of yeast MAD2, an essential component in the spindle checkpoint pathway that ensures metaphase is complete before anaphase begins. Combined immunolocalization of MAD2 and a recently cloned maize CENPC homologue indicates that MAD2 localizes
Yueming Liang et al.
Frontiers in microbiology, 11, 571209-571209 (2020-12-18)
phoD-harboring microorganisms facilitate mineralization of organic phosphorus (P), while their role in the regulation of soil P turnover under P-limited conditions in Pinus massoniana plantations is poorly understood. The aim of the present study was to investigate the effects of
Behnaz Akhoundi et al.
Experimental parasitology, 133(3), 307-313 (2013-01-02)
The diagnosis of visceral leishmaniasis (VL) in humans and animal reservoir hosts is difficult, particularly in rural areas where the disease is endemic and laboratory facilities are limited. This study aimed to develop a latex agglutination test (LAT) for the
Tina Histing et al.
Journal of investigative surgery : the official journal of the Academy of Surgical Research, 26(1), 30-35 (2013-01-01)
Osteoporosis is characterized by poor bone quality. However, it is still controversially discussed whether osteoporosis compromises fracture healing. Herein, we studied whether the course of healing of a femur fracture is affected by osteoporosis or age. Using the senescence-accelerated osteoporotic
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Protocolos

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

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