Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
581324-U
Ascentis® C18 (5 µm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
Sinónimos:
Ascentis RP18 HPLC Column
About This Item
Productos recomendados
Nombre del producto
Ascentis® C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Materiales
stainless steel column
Nivel de calidad
Agency
suitable for USP L1 (Similar to Phenomenex Luna C18)
Línea del producto
Ascentis®
Características
endcapped
fabricante / nombre comercial
Ascentis®
envase
1 ea of
Extensión del etiquetado
25% Carbon loading
Parámetros
≤70 °C temp. range
400 bar pressure (5801 psi)
técnicas
HPLC: suitable
LC/MS: suitable
L × D.I.
15 cm × 4.6 mm
superficie
450 m2/g
cobertura de la superficie
3.7 μmol/m2
impurezas
<5 ppm metals
Matriz
fully porous particle
silica gel high purity, spherical
grupo activo de la matriz
C18 (octadecyl) phase
tamaño de partícula
5 μm
tamaño de poro
100 Å
Intervalo de pH operativo
2-8
aplicaciones
food and beverages
técnica de separación
reversed phase
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Categorías relacionadas
Descripción general
Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.
Aplicación
- Preparation of polar embedded C18 stationary phase for efficient separation of peptides and proteins in high performance liquid chromatography.: This study details the development of a polar embedded C18 stationary phase, demonstrating its effectiveness in separating peptides and proteins. The Ascentis® C18 HPLC Column was utilized for its high separation efficiency, crucial for proteomics and biopharmaceutical applications (Ali et al., 2022).
- A dilute-and-shoot liquid chromatography-tandem mass spectrometry method for urinary 18-hydroxycortisol quantification and its application in establishing reference intervals.: Researchers used the Ascentis® C18 HPLC Column to develop a sensitive method for quantifying urinary 18-hydroxycortisol. This method aids in clinical diagnostics and endocrinological studies by providing accurate reference intervals (Zhao et al., 2022).
- A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS.: This comparative analysis utilized the Ascentis® C18 HPLC Column to separate and identify ginsenosides in American ginseng. The study highlights the column′s application in natural product research and quality control in the pharmaceutical industry (Hsu et al., 2022).
- Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Encapsulation Efficiency and In Vitro Release.: This research validates an HPLC method using the Ascentis® C18 Column to simultaneously quantify dexamethasone and melatonin in PLGA microspheres, offering a robust tool for drug delivery studies (Brugnera et al., 2022).
- Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method.: The Ascentis® C18 HPLC Column was employed to develop a stability-indicating method for analyzing ivermectin and clorsulon in pharmaceutical formulations, emphasizing its role in quality control and regulatory compliance (Padivitage et al., 2022).
Características y beneficios
- Excellent retention
- Symmetric peak shape
- High reproducibility
- Complete LC-MS compatibility
Información legal
Aplicación
Producto relacionado
cartucho de precolumna
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Artículos
Phosphonates analysis challenges due to their polar nature and lack of UV active chromophores in antiviral drugs.
Phosphonates analysis challenges due to their polar nature and lack of UV active chromophores in antiviral drugs.
Phosphonates analysis challenges due to their polar nature and lack of UV active chromophores in antiviral drugs.
Phosphonates analysis challenges due to their polar nature and lack of UV active chromophores in antiviral drugs.
Protocolos
Reversed-phase HPLC sometimes requires ion pairing for adequate retention of polar compounds due to hydrophobic interactions.
Reversed-phase HPLC sometimes requires ion pairing for adequate retention of polar compounds due to hydrophobic interactions.
Reversed-phase HPLC sometimes requires ion pairing for adequate retention of polar compounds due to hydrophobic interactions.
Reversed-phase HPLC sometimes requires ion pairing for adequate retention of polar compounds due to hydrophobic interactions.
Chromatograms
application for HPLCapplication for HPLCapplication for HPLCapplication for HPLC-
What flow rate should I use with Ascentis® Express HPLC Columns?
1 answer-
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Ascentis® Express HPLC Columns be used for LC-MS?
1 answer-
Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
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What is the storage solvent for Product 581324-U, Ascentis® C18 HPLC Column - 5 ?m particle size, L × I.D. 15 cm × 4.6 mm?
1 answer-
The storage solvent is a ratio of 40:60 water:acetonitrile for this column.
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