Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

U3383

Sigma-Aldrich

Urease Buffer Reagent

Sinónimos:

Urea colorimetric determination assay reagent

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Código UNSPSC:
12352204
NACRES:
NA.54

descripción

When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.

Nivel de calidad

200

Formulario

powder

mol peso

545 kDa

temp. de almacenamiento

2-8°C

Descripción general

Urease is a 545 kDa protein critical for the hydrolysis of urea to carbon dioxide and ammonia.
Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide. It is secreted by several bacterial species, invertebrates, fungi, and plants. Detection of urease activity is a potential diagnostic tool for Helicobacter pylori infections and several oral bacterial infections. two Ni2+ ions bridged by a hydroxyl group and a carbamylated lysine.

Aplicación

Urease Buffer Reagent has been used:
  • to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside method
  • in cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cells
  • to study the metabolic capacity of rat hepatocytes

Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea.

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H315 - H319 - H334

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000252832
SLCR3981
SLCL1109
SLCK2088
SLCH6287

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Kang Cheng et al.
Hepatology (Baltimore, Md.), 50(4), 1194-1203 (2009-07-29)
Tracking stem/progenitor cells through noninvasive imaging is a helpful means of assessing the targeting of transplanted cells to specific organs. We performed in vitro and in vivo studies wherein adult human hepatocytes and human fetal liver stem/progenitor cells were labeled
Gunnar Dahlén et al.
BMC oral health, 18(1), 89-89 (2018-05-20)
Urease is an enzyme produced by plaque bacteria hydrolysing urea from saliva and gingival exudate into ammonia in order to regulate the pH in the dental biofilm. The aim of this study was to assess the urease activity among oral
Yuta Enami et al.
Hepatology (Baltimore, Md.), 55(4), 1182-1192 (2011-10-14)
Organs from non-heart-beating donors are attractive for use in cell therapy. Understanding the nature of molecular perturbations following reperfusion/reoxygenation will be highly significant for non-heart-beating donor cells. We studied non-heart-beating donor rats for global gene expression with Affymetrix microarrays, hepatic
Cristian Follmer
Phytochemistry, 69(1), 18-28 (2007-08-21)
The broad distribution of ureases in leguminous seeds, as well as the accumulation pattern of the protein during seed maturation, are suggestive of an important physiological role for this enzyme. Since the isolation and characterization of jack bean urease by
L A Winkelman et al.
Journal of dairy science, 96(12), 7565-7577 (2013-10-15)
This study investigated the effect of 2 different types of long-acting insulin on milk production, milk composition, and metabolism in lactating dairy cows. Multiparous cows (n=30) averaging 88 d in milk were assigned to one of 3 treatments in a
Ver todos los artículos relacionados

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico