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S9626

Sigma-Aldrich

Sulfatase from Helix pomatia

Type H-1, sulfatase ≥10,000 units/g solid

Sinónimos:

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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About This Item

Número de CAS:
9016-17-5
Comisión internacional de enzimas:
3.1.6.1 (BRENDA, IUBMB)
Número CE:
232-772-1
Número MDL:
MFCD00132399
Código UNSPSC:
12352204
NACRES:
NA.54
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tpo

Type H-1

Nivel de calidad

200

Formulario

powder

sulfatase activity

≥10,000 units/g solid

secondary activity

≥300 unit/mg solid β-glucuronidase (at pH 5.0)

mol peso

~85 kDa by gel filtration

aplicaciones

diagnostic assay manufacturing

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

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Aplicación

Sulfatase from Helix pomatia has been used in:
  • sample extraction[1]
  • sulfatase assays[2]
  • enzymatic hydrolysis[3]
  • extract preparation for glucosinolate analysis[4]

Acciones bioquímicas o fisiológicas

Sulfatase from Helix pomatia facilitates the synthesis of thiohydroximates.[5] It causes the hydrolysis of β-naphthyl sulfate much faster than a-naphthyl sulfate and possesses regiospecificity in the hydrolysis of ortho and para substituted phenyl sulfates.[6]

Definición de unidad

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

sustrato

Referencia del producto
Descripción
Precios

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H317,H334

Clasificaciones de peligro

Resp. Sens. 1 - Skin Sens. 1

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000389811
0000381301
0000363885
0000377335
0000376660

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A single monooxygenase, ese, is involved in the metabolism of the organochlorides endosulfan and endosulfate in an Arthrobacter sp.
Weir K M, et al.
Applied and Environmental Microbiology, 72(5), 3524-3530 (2006)
Ivica Blažević et al.
Phytochemistry, 169, 112100-112100 (2019-11-28)
The glucosinolates (GSLs) is a well-defined group of plant metabolites characterized by having an S-β-d-glucopyrano unit anomerically connected to an O-sulfated (Z)-thiohydroximate function. After enzymatic hydrolysis, the sulfated aglucone can undergo rearrangement to an isothiocyanate, or form a nitrile or
Stephen S Hecht et al.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 13(6), 997-1004 (2004-06-09)
Vegetable consumption, including cruciferous vegetables, is protective against lung cancer, but the mechanisms are poorly understood. The purpose of this study was to investigate the effects of cruciferous vegetable consumption on the metabolism of the tobacco-specific lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)
Glucosinolate enhancement in cabbage induced by jasmonic acid application.
Fritz, V. A., Justen, V. L., Bode, A. M., Schuster, T., & Wang, M.
Hortscience: a Publication of the American Society For Horticultural Science Hortscience, 45, 1188-1191 (2010)
Use of diabetic test kits to assess the recovery of glucosinolates during isolation of canola protein
Ser W Y, et al.
Food Sci. Technol., 41(5), 934-941 (2008)
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Protocolos

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Questions

1–5 of 5 Questions  

  1. Can Sulfatase S9626 be dissolved in 0.2% NaCl?

    1 answer

    1. Solubility in 0.2% NaCl has not been tested for this product.

      Helpful?

  2. What is the solubility of Product S9626, Sulfatase from Helix pomatia?

    1 answer

    1. Prior to determining the activity of this enzyme, Sigma reconstitutes it in 0.2% (w/v) sodium chloride.

      Helpful?

  3. How is Product S9626, Sulfatase from helix pomatia, prepared?

    1 answer

    1. This enzyme is isolated by acetone fractionation.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. Does Product S9626, Sufatase from Helix pomatia, contain B-Glucuronidase activity?

    1 answer

    1. This product contains >300 units/mg solid B-Glucuronidase activity.

      Helpful?

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