P5813

Anti-p53 antibody, Mouse monoclonal

clone BP53-12, ascites fluid

• Sinónimos: Anti-TRP53, Anti-Transformation-related protein 53
• Número MDL: MFCD00164709
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: ascites fluid
• tipo de anticuerpo: primary antibodies
• clon: BP53-12, monoclonal
• mol peso: antigen 53 kDa
• contiene: 15 mM sodium azide
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using methacarn-fixed, paraffin-embedded sections of human malignant tissue; immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: suitable using cell lysates from human cell lines known to express high levels of the p53 protein
• isotipo: IgG2a
• Nº de acceso UniProt: P04637
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... TP53(7157)

Descripción

Descripción general

Monoclonal Anti-p53 (mouse Ig2a isotype) is derived from the BP53-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human wild-type p53 protein. The tumor suppressor gene TP53 (gene) codes for a transcription factor. TP53 gene is mapped to human chromosome 17p13.1. The human wild-type p53 protein is a 393 amino acid nuclear phosphoprotein, present in the nucleus of all normal mammalian cells.

Inmunógeno

recombinant human wild-type p53 protein.

Aplicación

Monoclonal Anti-p53 antibody produced in mouse has been used in:

• western blotting
• immunohistochemistry
• immunocytochemistry
• enzyme linked immunosorbent assay (ELISA)

Mouse monoclonal clone BP53-12 anti-p53 antibody is a useful tool suitable for the detection and quantitation of p53 applying various immunochemical techniques, especially immunohistochemistry using conventionally fixed and processed histological sections. The product is also useful for immunoprecipitation, ELISA and immunocytochemical staining of cultured cell lines.

Acciones bioquímicas o fisiológicas

The tumor suppressor gene TP53 (gene) gene is involved in the regulation of cell cycle, senescence and apoptosis upon oncogene activation, stress and DNA damage. Point mutations of the wild-type gene have been suggested to be a key event in the development of malignancy as the mutant protein acts as a dominant oncogene. Indeed, mutations of the p53 gene are the most common molecular changes identified in human cancer. They have been reported to be a frequent feature of breast, lung, colon, ovarian, brain, testicular and bladder cancers, melanoma, neurofibrosarcoma, and certain types of leukemia. The accumulation of high levels of p53 is a potential novel marker for malignancy. Monoclonal Anti-p53 recognizes a denaturation resistant epitope on the primate p53 nuclear protein (53 kDa). It does not react with other cellular proteins.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable