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Merck

C0549

Sigma-Aldrich

Chloroform:Isoamyl alcohol 24:1

suitable for nucleic acid purification

Sinónimos:

chloroform-isoamyl alcohol

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About This Item

Código UNSPSC:
12190000
NACRES:
NA.52
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Formulario

liquid

contiene

0.6-1.0% ethanol as stabilizer

idoneidad

suitable for nucleic acid purification

aplicaciones

agriculture

Descripción general

Chloroform:Isoamyl Alcohol 24:1 is an organic reagent used for DNA extraction and precipitation.[1]

Aplicación

Chloroform:Isoamyl alcohol 24:1 has been used:

  • to extract bacterial genomic DNA, DNA from Daphnia spp, and salmon sperms.[2][3][4]
  • for a single DNA precipitation step for high-quality DNA extraction.[5]
  • for protein extraction and RNAse treatment.[5]
  • in phenolic oxidation to separate phenolics from the aqueous portion.[5]
  • for RNA isolation from brown adipose tissue (BAT), inguinal white adipose tissue (iWAT), and epididymal adipose tissue (eWAT).[6]
  • in modified cetyltrimethyl ammonium bromide (CTAB) method to extract total RNA from Impatiens balsamina.[7]

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

Órganos de actuación

Central nervous system, Liver,Kidney

Código de clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

185.0 °F

Punto de inflamabilidad (°C)

85 °C


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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species
Healey A, et al.
Plant methods, 10(1), 1-8 (2014)
A modified SDS-based DNA extraction method from raw soybean
Xia YC, et al.
Bioscience Reports, 39(2), BSR20182271-BSR20182271 (2019)
A modified CTAB-based protocol for total RNA extraction from the medicinal plant Impatiens balsamina (Balsaminaceae) for next-generation sequencing studies.
FOONG LC, et al.
Journal of Separation Science, 46(2) (2017)
Defective insulin-stimulated GLUT4 translocation in brown adipocytes induces systemic glucose homeostasis dysregulation independent of thermogenesis in female mice
Picatoste B, et al.
Molecular Metabolism, 53 (2021)
Harnessing microbially generated power on the seafloor.
Tender LM
Nature Biotechnology, 20(8), 821-825 (2002)

Contenido relacionado

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

Resumen de las técnicas habituales y las aplicaciones posteriores de la extracción y purificación del ADN genómico, el ADN plasmídico y el ARN total de células, tejidos, sangre, virus y otros tipos de muestra.

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Questions

1–2 of 2 Questions  
  1. Hi, what are the storage instructions for this product?

    1 answer
    1. This product may be stored at ambient temperature, tightly sealed, in a well-ventilated place.

      Helpful?

  2. Hello, what is the volume of this mixture please? And what is its shelf-life? Thanks. Katy

    1 answer
    1. This product is available in two package sizes, 1 pint (473 mL) and 1 quart (946 mL).

      This product is not assigned an expiration date or recommended retest date. Customers should routinely inspect products with no expiration date or recommended retest date to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services

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