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Merck

P2850

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 1

DMSO solution, mixture of inhibitors

Sinónimos:

Phosphatase Inhibitor

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About This Item

EC Number:
UNSPSC Code:
12352200
NACRES:
NA.54

product name

Phosphatase Inhibitor Cocktail 1, DMSO solution

form

DMSO solution

Quality Level

shipped in

wet ice

storage temp.

2-8°C

General description

Phosphatase Inhibitor Cocktail 1 includes a mixture of inhibitors that will inhibit the L-isozymes of alkaline phosphatase as well as serine/threonine protein phosphatases such as PP1 and PP2A. This product contains microcystinLR, cantharidin, and (−)-p-bromotetramisole. Phosphatase Inhibitor Cocktail 1 has been replaced with P0044.

Application

Phosphatase Inhibitor Cocktail 1 has been used in nuclear protein isolation and electrophoretic mobility shift assay (EMSA) for catenin activation. It has also been used in western blot analysis.

Biochem/physiol Actions

Phosphatases are active regulators of a number of important biological processes. Inhibition of phosphatases by small molecules is useful to study its biological role.

Quantity

Recommended usage is a 100-fold dilution.

Analysis Note

Tested on cell extracts from animal tissues such as bovine liver, human placenta, and rabbit muscle, as well as on extracts from A431 and Jurkat cell lines.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

188.6 °F

flash_point_c

87 °C


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Discovery of protein phosphatase inhibitor classes by biology-oriented synthesis.
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Artículos

The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.

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