This product can be used in rat tissue homogenate. It already contains serine and cysteine protease inhibitors. Note that PMSF has a short half-life in aqueous solutions, specifically 55 minutes at 25°C and pH 7.5.
P8340
Protease Inhibitor Cocktail
Protease Inhibitor Cocktail
1 of 4 reviewers received a sample product or took part in a promotion
DMSO solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use with mammalian cell and tissue extracts, DMSO solution
Sinónimos:
Mezcla de inhibidor de proteasas
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About This Item
form:
DMSO solution
storage temp.:
−20°C
En este momento no podemos mostrarle ni los precios ni la disponibilidad
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Nombre del producto
Cóctel de inhibidores de proteasas, for use with mammalian cell and tissue extracts, DMSO solution
biological source
bovine lung
Quality Level
form
DMSO solution
storage temp.
−20°C
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General description
Cóctel de inhibidores de proteasas para extractos de células y tejidos de mamíferos, en disolución DMSO. Contiene seis componentes individuales para inhibir las proteasas de serina, cisteína, las proteasas ácidas y las aminopeptidasas.
Specificity
Inhibe las proteasas de serina y cisteína, las proteasas ácidas y las aminopeptidasas.
Application
Ideal para utilizar como componente del tampón durante el tratamiento con ultrasonidos, tampón de lisis y tampón para RIPA
Proporciona protección contra la actividad enzimática endógena en extractos celulares y de tejidos
Proporciona protección contra la actividad enzimática endógena en extractos celulares y de tejidos
Biochem/physiol Actions
Esta mezcla contiene componentes individuales, como la AEBSF a 104 mM, aprotinina a 80 μM, bestatina a 4 mM, E-64 a 1,4 mM, leupeptina a 2 mM y pepstatina A a 1,5 mM. Cada componente tiene propiedades inhibidoras específicas. La AEBSF y la aprotinina actúan para inhibir las proteasas de serina, como la tripsina, la quimiotripsina y la plasmina, entre otras. La bestatina inhibe las aminopeptidasas. La E-64 actúa contra las proteasas de cisteína. La leupeptina actúa contra las proteasas de serina y de cisteína. La pepstatina A inhibe las proteasas ácidas.
Features and Benefits
Inhibición de amplio espectro: inhibe múltiples clases de proteasas y aminopeptidasas
Conservación estable: se suministra como disolución en DMSO, lo que permite su conservación a largo plazo a -20°C
Rendimiento uniforme: disolución lista para usar
Conservación estable: se suministra como disolución en DMSO, lo que permite su conservación a largo plazo a -20°C
Rendimiento uniforme: disolución lista para usar
Components
AEBSF
Aprotinina
Bestatina
E-64
Leupeptina
Pepstatina A
Aprotinina
Bestatina
E-64
Leupeptina
Pepstatina A
Caution
Este cóctel debe conservarse a -20 °C.
Preparation Note
Se recomienda 1 ml de esta disolución combinada para la inhibición de las enzimas endógenas que se encuentran en 100 ml de lisado procedente de 20 g de hígado bovino o 10 ml de lisado de células CHO. Este producto se suministra como una disolución en DMSO.
also commonly purchased with this product
Referencia del producto
Descripción
Precios
related product
Referencia del producto
Descripción
Precios
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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Certificados de análisis (COA)
Lot/Batch Number
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Can this product be used for IL-6 detection using ELISA in rat tissue Homogenate? Can it be used along with PMSF without creating any problem in the assay?
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How long is the protease inhibitor cocktail stable at room temperature?
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This product ships without wet or dry ice and is stable for up to two weeks at ambient temperatures. The long-term stability of this material at room temperature has not been determined. The recommended long-term storage temperature is -20°C.
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Can we dilute it in water? The experiment I plan to do will require 100ul of Protease inhibitor per vial, and I have more than 50 samples to run.
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This product is ready to use and should be diluted into the lysis buffer.
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Is the protease cocktail P8340 supplied as a 100X solution or a 1X solution?
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This cocktail is essentially a 100X preparation. One milliliter of this solution is sufficient for the inhibition of endogenous enzymes found in 100 mL of lysate. Please see the link below to review this and additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/107/629/p8340dat-ms.pdfHelpful?
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What is the stock concentration of the Protease Inhibitor Cocktail P8340? Is it 1x or 100x?
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The concentration of inhibitors in this product is the following:AEBSF, 104 mMAprotinin, 0.085 mMBestatin, 4 mME-64, 1.4 mMLeupeptin, 2 mMPepstain A, 1.5 mM.
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Do we need to add PMSF as well along with this cocktail? I intend to use this for RIPA buffer
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PMSF is a serine protease inhibitor. You can certainly add along with the cocktail if needed.
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What is the working concentration if adding to the blood?
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The recommended amount to use is 1:100 (v:v) directly to the whole blood, but one has to take into consideration that this will disrupt the coagulation cascade. If it is a concern, the protease inhibitor cocktail should be added after separation of the blood components. In the serum use at the same concentration as for blood, 1:100 (v:v)
There are articles citing this product when using blood samples. 1-100 was used in both articles:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346264/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2722770/Helpful?
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How long can this product be stored at -20C?
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This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 4 years after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. The retest date can be found in the lot specific Certificate of Analysis. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.
Please see the link below to review a sample Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P8340-1ML0000227406.pdfHelpful?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product P8340, Protease Inhibitor Cocktail, be used with Ezview Protein A and Anti FLAG M2 affinity gels.
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Yes, this product can be used with both Ezview Protein A and Anti FLAG M2 affinity gels.
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