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Merck

P0372

Sigma-Aldrich

Anti-Podocin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Podocin Antibody, Podocin Antibody - Anti-Podocin antibody produced in rabbit

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

mol peso

antigen ~42 kDa (doublet)

reactividad de especies

human, rat, mouse

técnicas

indirect immunofluorescence: 10-20 μg/mL using acetone-fixed human or rat kidney frozen sections
western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat glomeruli

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... NPHS2(7827)
mouse ... Nphs2(170484)
rat ... Nphs2(170672)

Descripción general

Podocin is a podocyte structural protein, that belongs to the band-7-stomatin family of lipid raft-associated proteins. This family contains more than 1800 conserved proteins which share ∼150 amino acid domains showing similarity to mitochondrial protein prohibitin (PHB).
Podocin is a hairpin-like integral membrane protein with intracellular N- and C- termini. Podocin is located at the insertion site of the slit membrane, an intercellular junction found in mammalian kidney.

Especificidad

Anti-Podocin recognizes human, mouse, and rat podocin.

Inmunógeno

Synthetic peptide corresponding to amino acid residues of human podocin with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in rat.

Aplicación

Anti-Podocin antibody produced in rabbit has been used for Western Blotting and immunohistochemistry.

Acciones bioquímicas o fisiológicas

Podocin and nephrin are essential for maintaining the slit diaphragm. Studies show that mutations in this gene account for around 15% cases of nephrotic syndrome cases. Podocin interacts with cholesterol and promotes the formation of protein-lipid supercomplexes, which control ion channel complexes. The podocin gene is known to be associated with autosomal recessive SRNS (nephrotic syndrome type 2). Podocin actively participates in the ultrafiltration process occuring in kidney.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Los clientes también vieron

Induction of podocyte VEGF164 overexpression at different stages of development causes congenital nephrosis or steroid-resistant nephrotic syndrome.
Veron D et al
The American Journal of Pathology, 177(5), 2225-2233 (2010)
Batoul Wehbi et al.
Journal of the American Society of Nephrology : JASN, 30(7), 1238-1249 (2019-06-23)
IgA nephropathy (IgAN) often follows infections and features IgA mesangial deposition. Polymeric IgA deposits in the mesangium seem to have varied pathogenic potential, but understanding their pathogenicity remains a challenge. Most mesangial IgA1 in human IgAN has a hypogalactosylated hinge
The SPFH domain-containing proteins: more than lipid raft markers.
Browman D T, et al.
Trends in Cell Biology, 17(8), 394-402 (2007)
Excess podocyte semaphorin-3A leads to glomerular disease involving plexinA1-nephrin interaction.
Reidy KJ et al
The American Journal of Pathology, 183(4), 1156-1168 (2013)
NPHS2 mutations account for only 15% of nephrotic syndrome cases.
Guaragna MS et al
BMC Medical Genetics, 16(88) (2015)

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