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M9309

Sigma-Aldrich

McCoy′s 5A Medium

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

5A basal medium

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About This Item

MDL number:
MFCD00217560
UNSPSC Code:
12352207
NACRES:
NA.71

product name

McCoy′s 5A Medium, Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 2.2 g/L
L-glutamine: 0.21 g/L
phenol red: 0.011 g/L
glucose: 3.0 g/L (Dextro)

shipped in

ambient

storage temp.

2-8°C

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General description

The amino acid requirements for in vitro cultivation of Novikoff hepatoma cells were reported by McCoy and colleagues. These experiments were carried out using Basal Medium 5A, which was later modified to create McCoy′s 5A medium.

Application

McCoy′s 5A Medium has been used:
  • to culture HCT-116?cells
  • to culture clear cell renal cell carcinoma (ccRCC) cells Caki-1
  • to maintain and culture human bone osteosarcoma Saos-2 cell line for in vitro cell studies

Biochem/physiol Actions

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

also commonly purchased with this product

Referencia del producto
Descripción
Precios

supplement

Referencia del producto
Descripción
Precios

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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