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Merck

M6018

Sigma-Aldrich

4-Methylumbelliferyl β-D-cellobioside

glucanase substrate, fluorogenic, ≥98.00% (TLC), powder

Sinónimos:

4-Methylumbelliferyl beta-D-cellobioside

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About This Item

Fórmula empírica (notación de Hill):
C22H28O13
Número de CAS:
Peso molecular:
500.45
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

product name

4-Methylumbelliferyl β-D-cellobioside, glucanase substrate

Quality Level

assay

≥98.00% (TLC)

form

powder

solubility

water: 19.60-20.40 mg/mL, clear, colorless to faintly yellow

storage temp.

2-8°C

SMILES string

CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15-,16+,17-,18-,19-,20-,21-,22+/m1/s1

InChI key

PRTGXBPFDYMIJH-MKQZUAMYSA-N

Application

4-Methylumbelliferyl β-D-cellobioside has been used as a substrate:
  • to assay cellulase from A. niger
  • to determine the extracellular enzyme activity for 1,4-β-cellobiohydrolase
  • to assay the activity of bound enzyme, endoglucanase I (EGIc) Trichoderma reesei class II hydrophobins (HFBI)

Biochem/physiol Actions

4-Methylumbelliferyl β-D-cellobioside is used as a substrate for cellulase assays. It is mainly used for activity staining of cellulases in polyacrylamide gels.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

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Methylxanthine Drugs Are Chitinase Inhibitors: Investigation of Inhibition and Binding Modes
Rao FV, et al.
Chemistry & Biology, 12(9), 973-980 (2005)
Surface adhesion of fusion proteins containing the hydrophobins HFBI and HFBII from Trichoderma reesei
Linder M, et al.
Protein Science, 11(9), 2257-2266 (2002)
Reconciling opposing soil processes in row-crop agroecosystems via soil functional zone management
Williams A, et al.
Agriculture, Ecosystems & Environment, 236, 99-107 (2017)
G P Xue et al.
Journal of general microbiology, 138(11), 2397-2403 (1992-11-01)
A plant polysaccharide hydrolase cDNA, designated celD, was isolated from a cDNA library of the rumen fungus Neocallimastix patriciarum. The enzyme encoded by celD had endoglucanase, cellobiohydrolase and xylanase activities. Deletion analysis revealed that celD cDNA can be truncated to
A V Gusakov et al.
Biochimica et biophysica acta, 1073(3), 481-485 (1991-04-09)
Using 4-methylumbelliferyl (MUF) beta-D-cellobioside as a substrate, the ability of cellobiohydrolase I from Trichoderma longibrachiatum to catalyze transglycosylation has been demonstrated. At substrate concentrations greater than 2 mM, the formation of MUF-tetrasaccharide was detected using HPLC. In the course of

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