L8275

Lectin from Galanthus nivalis (snowdrop)

lyophilized powder

• Sinónimos: Galanthus nivalis lectin, GNL
• MDL number: MFCD00163718
• UNSPSC Code: 12352202
• NACRES: NA.32

Propiedades

• form: lyophilized powder
• Quality Level: 200
• potency: <20 μg per mL agglutination activity
• composition: Protein, ≥90% Lowry
• impurities: salt, essentially free
• storage temp.: 2-8°C

Descripción

General description

Lectin from Galanthus nivalis (snowdrop) is a tetrameric, 12 kDa mannose binding lectin. It belongs to the family, Amaryllidacea. It is specific to α-1,3- or 1,6- linked D-mannose of carbohydrates. Snowdrop lectins are toxic to insects.

Application

Lectin from Galanthus nivalis (snowdrop) has been used:

• to coat ELISA plates
• as reverse transcriptase inhibitor
• in ELISA for detection of envelope glycoproteins of the human and simian immunodeficiency viruses

Biochem/physiol Actions

Lectin from Galanthus nivalis (snowdrop) provide resistance against pests and are used in transgenic plants. Snowdrop lectin functions as a carrier to transport fused proteins to the hemolymph as it crosses gut epithelium of target species to exhibit insecticidal effects.

GNL agglutinates rabbit erythrocytes; human erythrocytes are not agglutinated. GNL sugar specificity is directed to the nonreducing end of the terminal α-D-mannosyl residue of glycoconjugates.

Preparation Note

Purified by affinity chromatography according to Kaku.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilution in phosphate buffered saline, pH 7.2 of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of rabbit erythrocytes after 1 hr incubation at 25 °C.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)