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Merck
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Key Documents

L6512

Sigma-Aldrich

Lectin from Helix pomatia

biotin conjugate, lyophilized powder

Sinónimos:

Helix pomatia agglutinin, HPA

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

conjugate

biotin conjugate

form

lyophilized powder

extent of labeling

2-4 mol biotin per mol protein

impurities

salt, free

storage temp.

−20°C

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General description

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. These oligomeric proteins are characterized by the presence of a carbohydrate recognition domain.

Application

Lectin from Helix pomatia has been used to study its binding activities in Gyrodactylus derjavini parasitizing fins of Oncorhynchus mykiss.

Biochem/physiol Actions

Lectin is useful in glycoconjugate characterizing, imaging and targeting. Lectin participates in host recognition and tissue adhesion. Lectins shows resistance to proteolytic degradation in vivo.
HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Overview of fish immunity
Mucosal Health in Aquaculture, 3-54 (2015)
Histochemical characteristics of Gyrodactylus derjavini parasitizing the fins of rainbow trout (Oncorhynchus mykiss)
Buchmann K, et al.
Folia Parasitol (Praha), 45(4), 312-318 (1998)
Cédric Jäger et al.
BMC nephrology, 23(1), 178-178 (2022-05-11)
Recurrence of IgA nephropathy (IgAN) after kidney transplantation occurs in about 30% of patients. The relevance of recurrence for the long-term graft survival is expected to increase, since graft survival continues to improve. In a nested study within the Swiss
PEAS AND LENTILS
Encyclopedia of food sciences and nutrition, 4433-4440 (2003)
K Buchmann
Diseases of aquatic organisms, 32(3), 195-200 (1998-07-24)
A lethal effect of rainbow trout Oncorhynchus mykiss plasma containing intact complement factors on Gyrodactylus derjavini was demonstrated. It was associated with binding of complement factor C3 to certain carbohydrate-rich parasite structures. Parasites were exposed in vitro to plasma from
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