LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
L5542
LB Agar Plates
pre-poured LB agar plates for E. coli
Sinónimos:
pre-poured LB agar plates
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About This Item
Código UNSPSC:
41121800
NACRES:
NA.74
esterilidad:
sterile; autoclaved
idoneidad:
nonselective for Escherichia coli
nonselective for coliforms
nonselective for coliforms
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Productos recomendados
grado
for molecular biology
Nivel de calidad
esterilidad
sterile; autoclaved
Formulario
plates (10 cm)
plates (10cm, 20-25ml each)
capacidad
20-25 mL
temp. de almacenamiento
2-8°C
idoneidad
nonselective for Escherichia coli
nonselective for coliforms
Descripción general
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of Escherichia coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Aplicación
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
LB Agar Plates has been used:
LB Agar Plates has been used:
- to culture bacteria like, Escherichia coli strain INV- α and methicillin-susceptible Staphylococcus aureus[1]
- to culture numerous bacteria and is used in strain storage method[2]
- to analyse the egestion of midgut microbes during sand fly bites[3]
Características y beneficios
Lennox LB Agar plates provide:
- Convenient, ready-to-use 10cm format
- Standard formulation
Componentes
15g/L Agar
10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
13 - Non Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 answer-
Helpful?
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Will adding magnesium to the culture medium increase cell density?
1 answer-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What is the difference among the many LB - Lennox products?
1 answer-
LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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