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L0543
LB Agar Kanamycin-50, Plates
pre-poured LB agar plates with 50 ug/ml kanamycin
Sinónimos:
kanamycin agar plates
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About This Item
Código UNSPSC:
41121800
esterilidad:
sterile; autoclaved
idoneidad:
selective for Escherichia coli
selective for coliforms
selective for coliforms
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Productos recomendados
grado
for molecular biology
Nivel de calidad
esterilidad
sterile; autoclaved
Formulario
plates (10 cm)
plates (10cm, 20-25ml each)
capacidad
20-25 mL
temp. de almacenamiento
2-8°C
idoneidad
selective for Escherichia coli
selective for coliforms
Descripción general
Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Aplicación
Suitable for selective cultivation of E. coli strains that contain plasmids conferring kanamycin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins. LB Agar Kanamycin-50, Plates has been used in the screening of Agrobacterium tumefaciens[1] and E.coli JM83 strain with pGD103 plasmid.[2]
Características y beneficios
Miller′s LB Agar plates provide:
- Convenient, ready-to-use 10cm format
- Standard formulation plus antibiotic
Componentes
15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Deich RA, et al.
Journal of Bacteriology, 170(2), 489-498 (1988)
Artículos
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
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What is the Department of Transportation shipping information for this product?
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Why do the LB-agar plates with antibiotics have such a short expiration date?
1 answer-
The antibiotics in the LB-agar plates are not stable. The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate. Because of this, we recommend the plates are used as soon as possible once they are received. For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).
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