LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
L3272
LB Broth with agar (Luria low salt)
Powder microbial growth medium
Sinónimos:
Luria Agar Base (Miller’s Modification)
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About This Item
Código UNSPSC:
41106200
NACRES:
NA.85
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
grado
for molecular biology
Nivel de calidad
esterilidad
non-sterile
Formulario
powder
composición
Agar, 15 g/L
NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
técnicas
microbiological culture: suitable
pH
6.8-7.2(3.05% solution)
aplicaciones
food and beverages
microbiology
temp. de almacenamiento
room temp
idoneidad
nonselective for Escherichia coli
nonselective for coliforms
Categorías relacionadas
Descripción general
Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.
Aplicación
LB Broth with agar (Luria low salt) has been used:
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Características y beneficios
Luria′s low salt LB powder with agar provides:
- Easy scale-up using larger package sizes
- A budget-friendly alternative to pre-poured plates
- Standard formulation
Nota de preparación
1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.
Información legal
Zeocin is a registered trademark of Cayla Sarl
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Protocolos
General protocols for growth of competent cells in microbial medium.
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 answer-
Helpful?
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Will adding magnesium to the culture medium increase cell density?
1 answer-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
Helpful?
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What is the difference between the LB - Luria low salt products?
1 answer-
LB - Luria low salt is available in both powder (L3397) and powder with agar formats (L3272).
Helpful?
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