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GE17-0906-01

NHS Act Sepharose 4 Fast Flow

Cytiva 17-0906-01, pack of 25 mL

Sinónimos:

NHS-Activated Sepharose FF, pre-activated agarose matrix

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

N-hydroxysuccinimide activated groups

envase

pack of 25 mL

fabricante / nombre comercial

Cytiva 17-0906-01

condiciones de almacenamiento

(100% Isopropanol)

Matriz

4% cross-linked agarose

tamaño de partícula

45-165 μm

diámetro promedio

90 μm

cleaning in place

3-13(Ligand dependent)

ligand dependent

3-13

Intervalo de trabajo

3-12(3 (ligand dependent)-12 (ligand dependent))

idoneidad

suitable for bioprocess medium

temp. de almacenamiento

2-8°C

Descripción general

NHS-activated Sepharose 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides.

NHS-activated Sepharose 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups.

This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands.

NHS-activated Sepharose 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, NHS-activated Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Características y beneficios

  • NHS pre-activated medium for coupling of small amino-containing proteins and peptides in process-scale applications
  • High level of pre-activation gives a high degree of substitution of the selected ligand
  • NHS coupling method results in chemically stable ligand attachment
  • BioProcess medium supported for industrial applications

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

Sepharose is a trademark of Cytiva

Pictogramas

Exclamation mark

Palabra de señalización

Danger

Frases de peligro

Código de clase de almacenamiento

3 - Flammable liquids


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Artículos

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocolos

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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