G1N70

GenElute^™ Mammalian Genomic DNA Miniprep Kits

sufficient for 70 purifications

• Sinónimos: Mammalian Genomic DNA Miniprep, animal gDNA prep, gDNA miniprep, mammalian gDNA extraction, mammalian gDNA isolation, mammalian gDNA prep, mammalian gDNA purification, silica mammalian gDNA prep, Gen Elute
• Código UNSPSC: 41105501
• NACRES: NA.52

Propiedades

• uso: sufficient for 70 purifications
• Nivel de calidad: 200
• técnicas: DNA purification: suitable
• temp. de almacenamiento: 15-25°C

Descripción

Descripción general

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient method to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

The purified genomic DNA is ready for immediate use in downstream applications such as restriction digest, PCR, southern blots, and sequencing reactions.

Aplicación

The purified genomic DNA is ready for immediate use in downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• sequencing reactions
• cloning
• ligation

Características y beneficios

• Expected yield: 25 μg from 2 x 10⁶ cultured cells; 30 μg from 25 mg of tissue
• Elution volume: 200 - 400 μl
• Time required: 20 min after lysis
• A₂₆₀/A₂₈₀ ratio: 1.6 - 1.9
• Mechanical homogenization required: No

Principio

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Otras notas

For additional information, please see www.sigma-aldrich.com/genomicdna.

Información legal

GenElute is a trademark of Sigma-Aldrich Co. LLC

Los componentes del kit también están disponibles por separado

• C2112 Column Preparation Solution
• P2308 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
• R6148 RNase A solution

Información de seguridad

• Pictogramas: GHS08,GHS05,GHS07,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H290,H302,H315,H318,H334,H335,H410
• Consejos de prudencia: P234 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 8A - Combustible corrosive hazardous materials
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable