The decline in activity over time due to storage conditions has not been determined. Other Proteinase K products may ship at ambient temperature, indicating little or no degradation of the material is expected. However, this items ships on ice. Maintaining the shipping and storage temperature at -20°C is recommended for this product in order to assure optimum performance.
P2308
Proteinasa K from Tritirachium album
Proteinase K from Tritirachium album
lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
Sinónimos:
Endopeptidasa K
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About This Item
Número de CAS:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
grade
for molecular biology
Quality Level
product line
BioUltra
form
lyophilized powder
specific activity
≥30 units/mg protein
mol wt
28.93 kDa
composition
Protein, ≥90% biuret
impurities
≤1 ppm DNA
foreign activity
DNAse, Nickase and RNAse, none detected
shipped in
wet ice
storage temp.
−20°C
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Categorías relacionadas
Application
Útil para la inactivación proteolítica de nucleasas durante el aislamiento de ADN y ARN.
Útil para la inactivación proteolítica de nucleasas durante el aislamiento de ADN y ARN.
Elimina las endotoxinas que se unen a las proteínas catiónicas como la lisozima y la ribonucleasa A.
Se ha comunicado que es útil para el aislamiento de mitocondrias hepáticas, de levadura y de frijol mung
Determinación de la localización de las enzimas en las membranas
Tratamiento de cortes de tejido incluidos en parafina para exponer los sitios de unión de antígenos para el marcado con anticuerpos.
Digestión de proteínas de muestras de tejido cerebral para investigación de los priones en las encefalopatías espongiformes transmisibles (EET).
Elimina las endotoxinas que se unen a las proteínas catiónicas como la lisozima y la ribonucleasa A.
Se ha comunicado que es útil para el aislamiento de mitocondrias hepáticas, de levadura y de frijol mung
Determinación de la localización de las enzimas en las membranas
Tratamiento de cortes de tejido incluidos en parafina para exponer los sitios de unión de antígenos para el marcado con anticuerpos.
Digestión de proteínas de muestras de tejido cerebral para investigación de los priones en las encefalopatías espongiformes transmisibles (EET).
Biochem/physiol Actions
La proteinasa K es una serín proteasa estable y muy reactiva. La evidencia procedente de estudios de la estructura cristalina y molecular indica que la enzima pertenece a la familia subtilisina con una tríada catalítica en el sitio activo (ASP39-HIS69-ser224). Es estable en una amplia variedad de entornos: pH, sales amortiguadoras, detergentes (SDS) y temperatura. En presencia de SDS al 0,1 - 0,5 %, la proteinasa K conserva su actividad y digerirá una variedad de proteínas y nucleasas en preparaciones de ADN sin comprometer la integridad del ADN aislado.
Unit Definition
Una unidad hidrolizará la hemoglobina desnaturalizada en urea para producir color equivalente a 1,0 μmol de tirosina por minuto a pH 7,5 y a 37 °C (color por el reactivo Folin-Ciocalteu).
also commonly purchased with this product
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suggested gloves for splash protection
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Artículos
Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.
Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.
Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.
Pro K aids in disrupting cell membranes for DNA release, crucial for downstream molecular biology techniques.
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Hi, I'd like to know what happens if the lyophilized proteinase K (P238) is not stored at -20C for at least a week.
1 answer-
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Is P2308 designed for antigen unmasking during IHC, and is there literature supporting this application?
1 answer-
Proteinase K has not been tested for use with IHC. Product P2308 is specifically tested for use in molecular biology, particularly for preparing cell lysates and releasing nucleic acids, as well as for PCR applications. While the enzyme has been used to digest hair (keratin) and may have other applications, there are no guarantees that P2308 would be suitable for antigen retrieval. No literature references stating that Proteinase K is commonly used for IHC were found.
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Can P2308 be dissolved in water for nucleic acid preparation? If so, at what concentration? Thanks!
1 answer-
This product may be dissolved in water for nucleic acid preparations at 1 mg/mL.
Please see the link below to review the product datasheet which includes preparation instructions:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/116/320/p2308dat.pdfHelpful?
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How to prepare a solution of 20 mg/ml of pK (P2308-5MG)? In the section "Preparation Instructions" it mentions that I need to solve 1 mg in 1 ml of water. Does it means I'll have a final concentration of only 1 mg/ml?
1 answer-
The stock may also be prepared at 20 mg/ml concentration in 50 mM Tris-HCl, pH 8.0, containing 1 mM calcium chloride. This solution is stable for at least 12 months at 4°C. For additional information, please refer to the below publications where the product P2308 has been used.
1) https://www.nature.com/articles/s41598-017-17102-w#Sec9
2) https://www.frontiersin.org/articles/10.3389/fmicb.2018.00830/full#h3
3) https://elifesciences.org/articles/36187#s4Helpful?
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How to prepare Proteinase K solution to use in antigen retrieval of formalin fixed paraffin embedded tissue sections for the purpose of paraffin immunoflorescence? Which is the diluent of choice to prepare the solution from powder form of proteinase K.
1 answer-
This product is not tested for use in antigen retrieval. The product 21627, IHC Select Proteinase K protocol may be applied to this product, P2308. To prepare a stock solution, dissolve Proteinase K, P2308, at 0.2mg/mL in 0.05M Tris solution, pH 7.5.
For antigen retrieval in FFPA tissue sections (deparaffinized) the recommended dilution of the stock solution is 1:30.
End users would need to optimize final dilutionHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the recommended pH range for Product P2308, Proteinase K?
1 answer-
Proteinase K is normally used in the pH range of 7.5-8.0.
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How should I store solutions of Product P2308, Proteinase K?
1 answer-
Stock solutions in 40% glycerol of at least 20 mg/ml can be stored in 10 mM Tris-HCl buffer, pH 7.5.with 1 mM calcium acetate (see product P4850). Solutions are also stable at pH 8 at 4°C.
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What amount of Product P2308, Proteinase K, should I use for nucleic acid preparations?
1 answer-
Amounts for typical usage is 50-200 μg/mL.
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How should I prepare solutions of Product P2308, Proteinase K?
1 answer-
Solutions can be prepared in 25-50 mM Tris-HCl buffer, pH 8.0, containing 1-5 mM Calcium chloride.
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