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D6815

Sigma-Aldrich

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride

peroxidase substrate, liquid, suitable for membrane applications

Sinónimos:

DAB membrane substrate

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About This Item

MDL number:
MFCD00012969
UNSPSC Code:
12352204
PubChem Substance ID:
24894054
NACRES:
NA.83
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Nombre del producto

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride, for Membrane Applications

Quality Level

200

form

liquid

shipped in

wet ice

storage temp.

2-8°C

SMILES string

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

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Application

3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride has been used as a substrate for peroxidase coupled secondary antibody.
  • in paraffin heart sections of mice for immunohistochemical and immunofluorescent staining[1]
  • in immunotransfer of salivary protein of laboratory Lutzomyia ovallesi[2]
  • in immunoperoxidase staining of lung tissue[3]
The DAB (3,3′-Diaminobenzidine tetrahydrochloride) Two-component Enhanced Liquid Substrate System is a precipitating substrate system for peroxidase. DAB Two-component Liquid Substrate System produces a brown precipitate for use in immunohistological and immunoblotting staining procedures.

Biochem/physiol Actions

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase. This feature is exploited for visualization in histochemical (HC) and immunohistochemical (IHC) staining.[4]

Packaging

Set consists of 4 x 100 ml Solution A and 15 ml Solution B.

Caution

Stable for up to 18 mo. when stored at 2-8 °C in the original container.

Quantity

Each DAB Two-component Liquid Substrate System will provide 500 mL of the DAB substrate solution.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • 3, 3′-Diaminobenzidine enhanced liquid substrate system 4 x 100

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H341,H350

Hazard Classifications

Carc. 1B - Muta. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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Certificados de análisis (COA)

Lot/Batch Number
0000280820
SLCQ9651
0000280820
SLCQ9651
SLCG5072

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Visite la Librería de documentos

Sandfly saliva of Lutzomyia ovallesi (Diptera: Psychodidae) as a possible marker for the transmission of Leishmania in Venezuela Andes region
Nieves E, et al.
Journal of Vector Borne Diseases, 49, 8-8 null
Sandfly saliva of Lutzomyia ovallesi (Diptera: Psychodidae) as a possible marker for the transmission of Leishmania in Venezuela Andes region
Nieves E, et al.
Journal of Vector Borne Diseases, 49(1), 8-8 (2012)
Acute erythropoietin cardioprotection is mediated by endothelial response
Teng R, et al.
Basic Research in Cardiology, 106(3), 343-354 (2011)
Nanette Mittereder et al.
Journal of immunology (Baltimore, Md. : 1950), 197(2), 491-503 (2016-06-15)
ICOS, a member of the CD28 family, represents a key molecule that regulates adaptive responses to foreign Ags. ICOS is prominently expressed on T follicular helper (TFH) cells, a specialized CD4(+) T cell subset that orchestrates B cell differentiation within
Acute erythropoietin cardioprotection is mediated by endothelial response
Teng R, et al.
Basic Research in Cardiology, 106, 343-354 (2011)
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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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