D0819
DMEM - high glucose
StableCell™, with stable glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Sinónimos:
Medio de Eagle modificado de Dulbecco, glucosa elevada, DME, DMEM
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
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Nombre del producto
Dulbecco′s Modified Eagle′s Medium, With 4500 mg/L glucose, stable glutamine, and sodium bicarbonate, without sodium pyruvate., liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
suitability
suitable for
components
stable glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: no
glucose: high
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
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General description
This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Application
El medio de Eagle modificado de Dulbecco (DMEM) es una modificación del medio basal Eagle (BME) que contiene el cuádruple de concentraciones de aminoácidos y vitaminas. La formulación original contenía 1000 mg/l de glucosa y se utilizaba para cultivar células embrionarias de ratón. Desde entonces, se ha modificado de varias formas para soportar cultivos primarios de células de ratón y de pollo, así como una variedad de células normales y transformadas. Cada uno de estos medios ofrece una combinación diferente de L-glutamina y piruvato sódico. Además, los niveles de glucosa se han elevado a 4 500 mg/l, lo que contribuye al nombre «DMEM/High».
Legal Information
StableCell is a trademark of Sigma-Aldrich Co. LLC
also commonly purchased with this product
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Descripción
Precios
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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