NCI-H929

95050415, human unknown/unspecified, Lymphoblastoid

• Sinónimos: H929 Cell Line
• UNSPSC Code: 41106514

Propiedades

• product name: NCI-H929, 95050415
• biological source: human unknown/unspecified
• growth mode: Suspension
• karyotype: Near-tetraploid (8q+)
• morphology: Lymphoblastoid
• products: Immunoglobulin A (IgA)
• receptors: Transferrin, CD38
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human Caucasian IgA-producing plasmacytoma

Cell Line Description

This IgA-producing cell line has been established from a malignant effusion in a 62-year-old Caucasian woman with myeloma. They are positive for plasma cell antigen 1 (PCA-1), transferrin receptor and CD38 but negative for early B cell developmental markers. HLA DR and CALLA. NCI-H929 is negative for Epstein-Barr virus nuclear antigen. A rearrangement of the c-myc proto-oncogene has been observed as well as the expression of c-myc RNA and an activated ras allele.

Application

Chemotherapeutic agent studies, receptor studies, carcinogenesis assays.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 9,13
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8,11
vWA: 14,15

Culture Medium

RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 2-4 x 100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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