KARPAS-929

06072606, human bone marrow, round to polygonal cells growing in clusters in suspension

• Sinónimos: KARPAS 929, KARPAS, 929, KARPAS929
• UNSPSC Code: 41106514

Propiedades

• product name: KARPAS-929, from human bone marrow, 06072606
• biological source: human bone marrow
• growth mode: Suspension
• karyotype: Hyperdiploid with complex aberrant chromosomes
• morphology: Round to polygonal cells growing in clusters in suspension
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice

Descripción

Cell Line Origin

Human multiple myeloma

Cell Line Description

Established in 1984 from the bone marrow of a 67-year old woman diagnosed with myeloma who survived for one year after diagnosis.

DNA Profile

Not specified

Culture Medium

RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS)

Subculture Routine

Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 10⁵ cells/ml; 5% CO₂; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 10⁵ cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 10⁵ cells/ml, this tends to equate to a 1:3 split every 2-3 days.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.