L-M (TK-)

90083001, mouse connective tissue, Fibroblast

• UNSPSC Code: 41106514

Propiedades

• product name: L-M (TK-), 90083001
• biological source: mouse connective tissue
• growth mode: Adherent
• karyotype: Modal no. 45; hyperdiploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse C34/An connective tissue

Cell Line Description

Derived from a sub-line of a BUdR resistant strain of the L-M mouse fibroblast cell line. This cell line has potential for use in genetic and biochemical studies including somatic cell hybridisation and gene transfer.

Application

Gene transfer, genetic, biochemical and hybridisation studies

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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