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Key Documents

B1059

Sigma-Aldrich

Bcl-w (Minus C-Terminus) human

>95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

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About This Item

MDL number:
MFCD03846039
UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

200

assay

>95% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

18 kDa by SDS-PAGE

UniProt accession no.

Q92843

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... BCL2L2(599)

General description

This recombinant Bcl-w lacks the mitochondrial targeting sequence but maintains the ability to neutralize pro-apoptotic Bcl-2 family members.

Biochem/physiol Actions

Like Bcl-2 and Bcl-x, the Bcl-w protein promotes cell survival, in contrast to other close homologues, Bax and Bak, which facilitate cell death. Bcl-w is an anti-apoptotic member of the Bcl-2 family that prevents release of cytochrome c from the mitochondrial intermembrane space into the cytosol. It is required for normal sperm maturation. Natural Bcl-w contains a C-terminal mitochondrial targeting sequence.

Physical form

Supplied as a 0.2 μm filtered solution in 25 mM HEPES, pH 7.5, 0.1 M KCl, and 10% glycerol.

Analysis Note

Measured by its ability to inhibit caspase-8-cleaved human BID-mediated release of cytochrome c from isolated mouse liver mitochondria using ELISA.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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W Yan et al.
Molecular endocrinology (Baltimore, Md.), 14(5), 682-699 (2000-05-16)
Bcl-w, a prosurvival member of the Bcl-2 family, is essential for spermatogenesis. However, the mechanisms by which Bcl-w participates in the regulation of apoptosis in the testis are largely unknown. To explore the potential role of Bcl-w in the regulation
The proto-oncogene Bcl-2 and its role in regulating apoptosis.
G Kroemer
Nature medicine, 3(6), 614-620 (1997-06-01)
Sajid Khan et al.
Nature medicine, 25(12), 1938-1947 (2019-12-04)
B-cell lymphoma extra large (BCL-XL) is a well-validated cancer target. However, the on-target and dose-limiting thrombocytopenia limits the use of BCL-XL inhibitors, such as ABT263, as safe and effective anticancer agents. To reduce the toxicity of ABT263, we converted it
BCL-2 family members and the mitochondria in apoptosis.
A Gross et al.
Genes & development, 13(15), 1899-1911 (1999-08-13)
Yonghan He et al.
Nature communications, 11(1), 1996-1996 (2020-04-26)
Small molecules that selectively kill senescent cells (SCs), termed senolytics, have the potential to prevent and treat various age-related diseases and extend healthspan. The use of Bcl-xl inhibitors as senolytics is largely limited by their on-target and dose-limiting platelet toxicity.

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