A9309
Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Sinónimos:
HRP Goat Anti-Mouse IgG (Fc specific)
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About This Item
Productos recomendados
origen biológico
goat
Nivel de calidad
conjugado
peroxidase conjugate
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
secondary antibodies
clon
polyclonal
Formulario
buffered aqueous solution
técnicas
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot: 1:40,000-1:80,000 using total cell extract of HeLa cells
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
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Descripción general
Goat polyclonal Mouse IgG (Fc specific)–Peroxidase antibody is determined against normal mouse serum, mouse IgG (whole molecule), the Fc fragment of mouse IgG and the Fab fragment of mouse IgG, the conjugate is specific for mouse IgG and shows no reaction with the Fab fragment of mouse IgG. The conjugate only shows reactivity with mouse IgG (whole molecule) and the Fc fragment of mouse IgG, when tested in ELISA. The conjugate shows no reaction with the Fab fragment of mouse IgG, human IgG, IgA, IgM, or rat IgG in ELISA.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum.
Aplicación
Anti-Mouse IgG (Fc specific)-Peroxidase antibody has been used:
- in immunoblotting
- in immunohistochemistry
- in enzyme linked immunosorbent assay (ELISA)
- in agar block precipitin titration
- in western blotting
- in assessment of in vitro poly(ADP-ribose)polymerase (PARP) activity
Indirect ELISA was performed on sera from mice immunized to SV40-Tag using HRP-conjugated goat anti-mouse Fc specific IgG. Antibody was used at a 1:1000 dilution for 30 minutes at 37 degrees.
Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.
Acciones bioquímicas o fisiológicas
IgG antibodies help in neutralizing the effects caused by viruses and toxins.
IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu™ Red into detectable chromophores, light-emitters or fluorescers, respectively.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu™ Red into detectable chromophores, light-emitters or fluorescers, respectively.
Otras notas
Antibody adsorbed with human IgG and rat serum proteins.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.
Nota de preparación
Adsorbed to reduce background staining with human or rat samples.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Información legal
Ampliflu is a trademark of Sigma-Aldrich Co. LLC
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Skin Sens. 1
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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