SCC048
N27 Rat Dopaminergic Neural Cell Line
Rat
Sinónimos:
Dopamine Neural Cells, 1RB3AN27 Cell Line
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81
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product name
N27 Rat Dopaminergic Neural Cell Line, The N27 rat dopaminergic neural cell line has been used as a dopaminergic neuron model for in vitro and in vivo studies.
biological source
rat
Quality Level
technique(s)
cell culture | stem cell: suitable
shipped in
liquid nitrogen
General description
Parkinson’s disease is a neurodegenerative disorder of the central nervous system that affects more than 6 million people worldwide. The motor symptoms of Parkinson′s disease result from the death of dopamine generating cells in the substantia nigra, a region of the midbrain. Numerous efforts have been made to slow the loss or replace these dopamine producing neurons in vivo.
The N27 rat dopaminergic neuron cell line was harvested from E12 rat mesencephalic tissue and was transfected with SV40 to immortalize the cell line. The N27 cell line, when injected into the striata of 6-hydroxydopamine-lesioned rats (an animal model of PD) caused a time-dependent improvement in neurological deficits. This immortalized cell line has been carefully characterized in studies of dopamine biosynthesis, neurotoxicity and used as a dopaminergic neuron model for in vitro and in vivo studies.
Cell Line Description
Neural Stem Cells
Neural Lineage Cells
Neural Lineage Cells
Application
Research Category
Stem Cell Research
Neuroscience
Stem Cell Research
Neuroscience
Research Sub Category
Neural Stem Cells
Neurochemistry & Neurotrophins
Neurodegenerative Diseases
Neuroregenerative Medicine
Neural Stem Cells
Neurochemistry & Neurotrophins
Neurodegenerative Diseases
Neuroregenerative Medicine
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
Quality
• Each vial contains ≥ 1X106 viable cells.
• Cells are negative for mycoplasma contamination.
• Cells are negative for mycoplasma contamination.
Storage and Stability
N27 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
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