MAB1254

Anti-Chymase Antibody, clone B7

clone B7, 2.4 mg/mL, Chemicon^®

• Sinónimos: Mast Cell Protease I
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: B7, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• concentration: 2.4 mg/mL
• technique(s): dot blot: suitable; immunohistochemistry: suitable
• isotype: IgG1κ
• suitability: not suitable for Western blot
• NCBI accession no.: NM_001836.2
• UniProt accession no.: P23946
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CMA1(1215)

Descripción

General description

Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.

Specificity

Reacts with human mast cell chymase.

Immunogen

Chymase purified from human skin.

Application

Anti-Chymase Antibody, clone B7 is an antibody against Chymase for use in DB, IH.

Dot blotting: 0.5-1.0 μg/mL.

Immunohistochemistry: 0.5-1.0 μg/mL. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.

Not recommended for use on Western blots.

Optimal working dilutions must be determined by end user.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

Physical form

Format: Purified

Purified by protein G affinity chromatography. Liquid in PBS with 20% glycerol, pH 7.4.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. Antibody may also be stored at -20ºC in undiluted aliquots.

Analysis Note

Control
POSITIVE CONTROL: lung, heart, skin, placenta.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable