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Merck
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Key Documents

71296

Millipore

PhosphoSafe Extraction Reagent

Novagen′s PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state.

Sinónimos:

Protein Extraction Reagent

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About This Item

UNSPSC Code:
41106510
NACRES:
NA.77

Quality Level

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

immunoblotting: suitable

application(s)

sample preparation

shipped in

dry ice

storage temp.

−70°C

General description





PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. This reagent contains the same formula as CytoBuster Protein Extraction Reagent (Trim 2001), but also includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, β-glycerophosphate, and sodium pyrophosphate. PhosphoSafe Extraction Reagent is compatible with kinase assays, protein interaction analysis, and other applications. This reagent is provided in convenient 25-ml bottles (1 bottle for 71296-3, 5 bottles for 71296-4).

Features and Benefits

  • Phosphorylation state preserved during extraction
  • Compatible with kinase assays

Warning

Toxicity: Toxic (F)

Other Notes

Trim, J.E. and Sawyer, D.L. 2001. inNovations12, 5.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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