1.08218

Trifluoroacetic acid solution

(25% solution in water) for protein sequenation

• Sinónimos: Trifluoroacetic acid solution, TFA solution, TFA
• Fórmula lineal: CF₃COOH
• Número de CAS: 76-05-1
• Peso molecular: 114.02
• Beilstein/REAXYS Number: 5446934
• MDL number: MFCD00004169
• UNSPSC Code: 12352106
• NACRES: NB.21

Propiedades

• Quality Level: 200
• form: liquid
• composition: Water, 74.5-75.5% Karl Fischer
• concentration: 24.5-25.5% (trifluoroacetic acid)
• density: 1.12 g/cm³ at 20 °C
• storage temp.: 15-25°C
• SMILES string: OC(=O)C(F)(F)F
• InChI: 1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)
• InChI key: DTQVDTLACAAQTR-UHFFFAOYSA-N

Descripción

Application

Trifluoroacetic acid (TFA) can be used as a solvent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).

Analysis Note

Assay (alkalimetric): 24.5 - 25.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %

Información de seguridad

• pictograms: GHS05
• signalword: Danger
• hcodes: H314 - H412
• pcodes: P273 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A
• Storage Class: 8B - Non-combustible, corrosive hazardous materials
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable