MABS2005
Anti-2A peptide Antibody, clone 3H4
clone 3H4, from mouse
Synonym(s):
Anti-2A Antibody
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
3H4, monoclonal
species reactivity
mouse
packaging
antibody small pack of 25 μg
technique(s)
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
shipped in
ambient
target post-translational modification
unmodified
General description
2A peptides and 2A-like peptide sequences (known as cis-acting hydrolase elements or CHYSEL) are a superior alternative to internal ribosomal entry sites (IRES) for coordinating the expression of multiple gene products from a single recombinant construct. The 2A sequences are relatively short peptides of about 20 amino acids (depending on the virus of origin) containing the consensus motif Asp-Val/Ile-Glu-X-Asn-Pro-Gly-Pro. 2A peptides allow multiple proteins to be encoded as polyproteins, which then dissociate into component proteins upon translation. The 2A sequence impairs normal peptide bond formation via a mechanism called ribosomal skipping, which results in effective, non-enzymatic generation of distinct peptide products from a single multicistronic construct. 2A peptides are used by several families of viruses, the best known foot-and-mouth disease virus of the Picornaviridae family, for producing multiple polypeptides. Anti-2A peptide, clone 3H4 recognizes 2A sequence derived from foot and mouth piconavirus (VKQTLNFDLLKLAGDVESNPG*P) with cleavage site between G and P. (Ref.: Trichas, G, et al. (2008). BMC Biol. 6: 40).
Specificity
Clone 3H4 detects 2A peptide in murine cells.
Immunogen
KLH-conjuated linear peptide corresponding to sequence CGDVEENPG (free C-term) derived from Thosea asigna virus.
Application
Anti-2A peptide, clone 3H4, Cat. No. MABS2005, is a mouse monoclonal antibody that detects 2A peptide and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Western Blotting Analysis: A representative lot detected 2A peptide in HEK293 SpCas9-P2A-Puro and NIH3T3 SpCas9-T2A-Puro cell lysates (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Immunoprecipitation Analysis: A representative lot detected 2A peptide in HeLa cells expressing SpCas9-P2A or plain HeLa cells, HEK293 cells expressing SpCas9-T2A or plain HEK293 cells (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Immunofluorescence Analysis: A representative lot detected 2A peptide in HeLa cells transiently transfected with SaCas9-T2A-GFP (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Immunoprecipitation Analysis: A representative lot detected 2A peptide in HeLa cells expressing SpCas9-P2A or plain HeLa cells, HEK293 cells expressing SpCas9-T2A or plain HEK293 cells (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Immunofluorescence Analysis: A representative lot detected 2A peptide in HeLa cells transiently transfected with SaCas9-T2A-GFP (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Quality
Evaluated by Western Blotting in NIH3T3 expressing SpCas9-T2A-Puro cell lysate.
Western Blotting Analysis: 0.5 ug/mL of this antibody detected 2A peptide in 10 µg of NIH3T3 expressing SpCas9-T2A-Puro cell lysate.
Western Blotting Analysis: 0.5 ug/mL of this antibody detected 2A peptide in 10 µg of NIH3T3 expressing SpCas9-T2A-Puro cell lysate.
Target description
~150 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Certificates of Analysis (COA)
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