Prepared BSA solution products also available for purchase. These products have a shelf life ranging from 18 months to 2 years. Many are assigned a restest schedule with no firm expiration date. These products are prepared in saline, DPBS, or Tris. These solutions are filtered products and offered in a range of concentrations. The storage recommendation for these products is 2 - 8 deg. C. In addition, BSA is often used as a carrier protein for less stable compounds that are stored frozen. While there is no established shelf life for a BSA solution stored frozen, it is quite stable. See the link below for a sample Certificate of Analysis for one of the BSA solutions.
A7906
Bovine Serum Albumin
heat shock fraction, pH 7, ≥98%
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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50 G
$474.00
100 G
$683.00
500 G
$2,250.00
1 KG
$3,250.00
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50 G
$474.00
100 G
$683.00
500 G
$2,250.00
1 KG
$3,250.00
About This Item
Recommended Products
biological source
bovine
Quality Level
Assay
≥98%
form
lyophilized powder
mol wt
~66 kDa
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
blood typing: suitable
microbiological culture: suitable
impurities
≤5.0% Loss on drying
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT virus, none detected
VSV virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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Application
Bovine serum albumin has been used-
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Preparation Note
Prepared using heat shock fractionation.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
also commonly purchased with this product
recommended
Product No.
Description
Pricing
suggested gloves for splash protection
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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After reconstitution in PBS, for how long is the protein stable in solution if stored at 4°C or -20*C?
1 answer-
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Does this BSA have bovine immunoglobulins?
1 answer-
This product does contain bovine immunoglobulins. BSA products that do not contain immunoglobulins will state "globulin-free" or "essentially globulin-free" on the product detail page. Product A3156 is an example of an immunoglobulin free BSA product.
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Is Product A7906, Albumin from bovine serum (BSA), suitable for use as a blocking agent in ELISA?
1 answer-
We have not tested the use of A7906 in ELISA. Because the remainder of the product is mostly globulins, we would not suggest A7906 for ELISA use.
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Is Product A7906, Albumin from bovine serum (BSA), protease-free?
1 answer-
We have not tested A7906 in quality control to be protease-free. Other BSA products to suggest that are protease-free would be A3059 or B6917.
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Is Product A7906, Albumin from bovine serum (BSA), tested for insulin levels?
1 answer-
A7906 is not tested in quality control for insulin levels. One BSA that is indicated as "suitable for insulin RIA procedures" is A7888.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product A7906, Albumin from bovine serum (BSA), be used in immunohistochemistry?
1 answer-
We have not ourselves tested A7906 for use in immunohistochemistry. However, a literature search can turn up publications that cite this use, such as this paper: Surendran, K., et al., Wnt-Dependent Beta-Catenin Signaling Is Activated after Unilateral Ureteral Obstruction, and Recombinant Secreted Frizzled-Related Protein 4 Alters the Progression of Renal Fibrosis. J. Am. Soc. Nephrol., Vol. 16, pp. 2373-2384 (2005).
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