ZRB2059

Anti-MMP-7 Antibody, clone 2B3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Matrilysin (UniProt: P09237, also known as EC:3.4.24.23, Matrin, Matrix metalloproteinase-7, MMP-7, Pump-1 protease, Uterine metalloproteinase)
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2B3, recombinant monoclonal
• description: 2B3 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 29.68 kDa; observed mol wt ~25 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_002414
• UniProt accession no.: P09237
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... MMP7(4316)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2B3 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects MMP-7. It targets an epitope within 17 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of Human MMP-7.

Application

Quality Control Testing

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected MMP-7 in HT-29 cell lysate.

Tested Applications

Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected recombinant Human MMP-7.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MMP-7 in HT-29 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Matrilysin (UniProt: P09237; also known as EC:3.4.24.23, Matrin, Matrix metalloproteinase-7, MMP-7, Pump-1 protease, Uterine metalloproteinase) is encoded by the MMP7 (also known as MPSL1, PUMP1) gene (Gene ID:  4316) in human. MMPs are a family zinc-dependent endoproteinases that degrade collagen and other structural proteins and are responsible for much of the turnover of matrix components. Each MMP contains a protease domain with a conserved HExxGHxxGxxHS/T sequence, where three histidine residues form a complex with a catalytic Zn atom.  They contain a generally conserved sequence PRCGVP around the chelating cysteine that is known as the “cysteine switch.” They are produced as zymogens, with a signal sequence and propeptide segment that must be removed during activation. The propeptide domain contains a conserved cysteine, which chelates the active zinc site. MMP-7 is a calcium and zinc-dependent endopeptidase that is synthesized with a signal peptide (aa 1-17) and a propeptide (aa 18-94) that are subsequently cleaved off to produce the active enzyme. It number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin, and proteoglycans. It is the smallest of all the MMPs and lacks the hemopexin-like domain that is common to other members of the family.  MMP-7 is widely expressed, and its elevated levels are observed in cycling endometrium, colorectal cancers and adenomas, hepatocellular carcinomas, pancreatic carcer, and in about 50% of gliomas. MMP-7 play a vital role in the maintenance of innate immunity in organs such as the lungs and intestines where it proteolytically activates anti-bacterial peptides such as pro-defensins. It is also required for mediating proteolytic release of TNF from macrophages. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Liao, H-Y., et al. (2021). Clin. Biochem. 92; 9-18; Ke, B., et al. (2017). Front. Physiol. 8; 21; Burke, B. (2004). Immunobiology. 209(1-2); 51-56).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable