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T3194

Sigma-Aldrich

Tetanus toxin

from Clostridium tetani, cleaves synaptobrevin (VAMP-2)

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About This Item

CAS Number:
676570-37-9
MDL number:
MFCD01095768
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Tetanus toxin from Clostridium tetani,

mol wt

150 kDa (heavy chain (100 kDa) and a light chain (50 kDa) connected through an interchain disulfide bond)

Quality Level

100

solubility

H2O: soluble

storage temp.

2-8°C

Gene Information

Clostridium tetani E88 ... tetX(1061100)

General description

Tetanus toxin is also known as tetanospasmin. It is a 150-kDa protein, produced by Clostridium tetani. This protein consists of a light and a heavy chain.

Application

Tetanus toxin from Clostridium tetani has been used as the exocytosis inhibitor/Golgi-dependent secretion to examine whether secretory carrier membrane protein (SCAMP5) stimulated α-synuclein secretion via the conventional Golgi-dependent pathway. It has also been used in peripheral blood mononuclear cells (PBMCs) culture for T cell proliferation screening.
Tetanus toxin from Clostridium tetani has been used to inhibit vesicular release in Pannexin 1-transfected Neuro2A cells1.

Biochem/physiol Actions

Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals. Internalized by receptor-mediated endocytosis and carried to spinal cord and brain by transsynaptic retrograde transport. Blocks the release of glycine from inhibitory interneurons of the spinal cord. Cleaves synaptobrevin (VAMP-2), and blocks synaptic vesicle exocytosis in vivo.
Tetanus toxin can cause spastic paralysis.

Preparation Note

Purified from cultures of Clostridium tetani by a modification of the method of Robinson, et al.

Reconstitution

Each vial, when reconstituted with 100 μL of sterile purified water, contains 25 μg of tetanus toxin in 20 mM HEPES, pH 7.4 with 1.25% lactose.

Analysis Note

Two bands by SDS-PAGE under reducing conditions (100 kDa heavy chain and 50 kDa light chain).

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Statements

H300 + H330,H370

Hazard Classifications

Acute Tox. 1 Oral - Acute Tox. 2 Inhalation - STOT SE 1

Target Organs

Central nervous system

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tetanus
Hunter's Tropical Medicine and Emerging Infectious Disease, 508-510 (2013)
Felix Horns et al.
Cell reports, 30(3), 905-913 (2020-01-23)
Antibody memory protects humans from many diseases. Protective antibody memory responses require activation of transcriptional programs, cell proliferation, and production of antigen-specific antibodies, but how these aspects of the response are coordinated is poorly understood. We profile the molecular and
MODELS vert The Tetanus Toxin Model of Temporal Lobe Epilepsy
Endocrine regulations, 804-807 (2009)
Secretory carrier membrane protein 5 is an autophagy inhibitor that promotes the secretion of alpha-synuclein via exosome
Yang Y, et al.
PLoS ONE, 12(7), e0180892-e0180892 (2017)
Rodolfo M Iglesias et al.
Neurochemical research, 37(6), 1355-1363 (2012-02-24)
Pannexin1 (Panx1), a protein related to the gap junction proteins of invertebrates, forms nonjunctional channels that open upon depolarization and in response to mechanical stretch and purinergic receptor stimulation. Importantly, ATP can be released through Panx1 channels, providing a possible
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