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Sigma-Aldrich

SNAPtide Botulinum Toxin A Substrate, Fluorogenic

≥90% (HPLC), lyophilized, FRET substrate, Calbiochem®

Synonym(s):

Botulinum Toxin Assay

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77

product name

SNAPtide Botulinum Toxin A Substrate, Fluorogenic, SNAPtide Botulinum Toxin A Substrate, Fluorogenic, is a synthetic peptide that acts as a FRET substrate with the fluorophore o-aminobenzoic acid and the acceptor chromophore 2,4-dinitrophenol (DNP).

Quality Level

100

Assay

≥90% (HPLC)

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

fluorescence

λex ~326 nm
λem ~423 nm

shipped in

ambient

storage temp.

2-8°C

General description

A synthetic peptide, fluorescence resonance energy transfer (FRET) substrate containing the N-terminally-linked fluorophore o-aminobenzoic acid (Abz) and the acceptor chromophore 2,4-dinitrophenol (DNP) linked to the C-terminus. The substrate is based on the native botulinum toxin type A cleavage site found in the synaptosome-associated protein SNAP-25. Cleavage of the substrate by botulinum toxin releases the fluorophore and full fluorescence is restored.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Substrate for the native botulinum toxin
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from water.

Preparation Note

Prepare a 5 mM stock solution of this peptide in DMSO as follows: Add 40 µl of DMSO to a vial containing 200 nmol of peptide.

Reconstitution

Following reconstitution, aliquot, cover with foil to protect from light, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Bigalke, H. and Shoer, L.F. 2000. In: Handbook of Experimental Pharmacology,145, Bacterial Protein Toxins (K. Aktories and I. Just, Eds.) pp. 407-443, Springer-Verlag, Berlin.
Schiavo, G., et al. 1993. J. Biol. Chem.268, 23784.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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