G3795
Anti-Gravin antibody, Mouse monoclonal
clone JP74, purified from hybridoma cell culture
Synonym(s):
Anti-A-Kinase Anchor Protein 12, Anti-AKAP12
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About This Item
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
JP74, monoclonal
form
buffered aqueous solution
species reactivity
mouse, rat, human
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.02-0.04 μg/mL using rat brain cytosolic S1 fraction
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... AKAP12(9590)
mouse ... Akap12(83397)
General description
Monoclonal Anti-Gravin (mouse IgG2a isotype) is derived from the hybridoma JP74 produced by the fusion of mouse myeloma cells (X63Ag8.653) and splenocytes from BALB/c mice immunized with fractionated membrane cortex proteins from human neurons [cell lines NT2-N and SK-N-BE(2)]. Gravin protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain.
Specificity
Monoclonal Anti-Gravin antibody is specific for human, rat and mouse gravin (approx. 300 kDa).
Immunogen
fractionated membrane cortex proteins from human neuron cell lines NT2-N and SK-N-BE(2).
Application
Anti-Gravin antibody has been used in western blotting immunofluorescence.
Monoclonal Anti-Gravin antibody is suitable for use in immunoblotting (using mpkCCD cells ). The product may also be used for immunocytochemistry and immunoprecipitation.
Biochem/physiol Actions
Gravin (or AKAP12) is a scaffolding kinase that functions as a tumor suppressor. This kinase interacts with PKC, PKA and other cell cycle signaling proteins to modulate cell division, growth, differentiation and inflammatory responses. The expression of gravin isoforms (α, β, and γ) is regulated by tissue-specific promoters. Gravin also acts as an autoantigen in myasthenia gravis patients .
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Jeffrey W Streb et al.
The Journal of biological chemistry, 279(53), 56014-56023 (2004-10-22)
A Kinase Anchoring Protein 12 (AKAP12; also known as src-suppressed C kinase substrate (SSeCKS) and Gravin) is a multivalent anchoring protein with tumor suppressor activity. Although expression of AKAP12 has been examined in a number of contexts, its expression control
Gravin regulates centrosome function through PLK1
Colicino E G, et al.
Molecular Biology of the Cell, 29(5), 532-541 (2018)
Irwin H Gelman
Frontiers in bioscience : a journal and virtual library, 7, d1782-d1797 (2002-07-23)
Scaffolding proteins are thought to facilitate the efficiency and specificity of enzyme/substrate reactions by coordinating their interaction along a cytoskeletal infrastructure in a spacial and temporal manner. Rodent SSeCKS, and its human orthologue, Gravin, seem to function as tumor suppressors
Sookkasem Khositseth et al.
Molecular & cellular proteomics : MCP, 10(1), M110-M110 (2010-10-14)
Previous studies in yeast have supported the view that post-transcriptional regulation of protein abundances may be more important than previously believed. Here we ask the question: "In a physiological regulatory process (the response of mammalian kidney cells to the hormone
J B Nauert et al.
Current biology : CB, 7(1), 52-62 (1997-01-01)
Subcellular targeting of protein kinases and phosphatases provides a mechanism for co-localizing these enzymes with their preferred substrates. A recently identified mammalian scaffold protein, AKAP79, controls the location of two broad-specificity kinases and a phosphatase. We have identified and characterized
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