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42085

Sigma-Aldrich

1-Fluoro-2,4-dinitrobenzene

purum p.a., ≥98.0% (GC)

Synonym(s):

2,4-Dinitro-1-fluorobenzene, DNFB, DNPF, FDNB, Sanger reagent

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50 G
NOK 1,190.00
250 G
NOK 4,470.00

NOK 1,190.00


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50 G
NOK 1,190.00
250 G
NOK 4,470.00

About This Item

Linear Formula:
(O2N)2C6H3F
CAS Number:
Molecular Weight:
186.10
Beilstein:
398632
EC Number:
MDL number:
UNSPSC Code:
12352100
PubChem Substance ID:
NACRES:
NA.22

NOK 1,190.00


Please contact Customer Service for Availability

Request a Bulk Order

grade

purum p.a.

Assay

≥98.0% (GC)

form

liquid

refractive index

n20/D 1.569 (lit.)

bp

178 °C/25 mmHg (lit.)

mp

25-27 °C (lit.)

density

1.482 g/mL at 25 °C (lit.)

cation traces

Ca: ≤50 mg/kg
Cd: ≤50 mg/kg
Co: ≤50 mg/kg
Cu: ≤50 mg/kg
Fe: ≤50 mg/kg
K: ≤100 mg/kg
Na: ≤100 mg/kg
Ni: ≤50 mg/kg
Pb: ≤50 mg/kg
Zn: ≤50 mg/kg

functional group

fluoro
nitro

storage temp.

2-8°C

SMILES string

[O-][N+](=O)c1ccc(F)c(c1)[N+]([O-])=O

InChI

1S/C6H3FN2O4/c7-5-2-1-4(8(10)11)3-6(5)9(12)13/h1-3H

InChI key

LOTKRQAVGJMPNV-UHFFFAOYSA-N

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General description

1-Fluoro-2,4-dinitrobenzene(FDNB, Sanger′s reagent) is used to identify the amino acid sequence. Thischemical is used to label the free amino groups in proteins and peptides. Itcan also be used to protect the amine group, and for phenol deoxygenation. Additionally,it is a very helpful reagent for nucleophilic substitution reactions tointroduce a chromophore group to amines, phenols, and thiols.

Application

1-Fluoro-2,4-dinitrobenzene is used as a derivatization agent:
  • For aminoglycoside compounds such as amikacine, tobramycine, gentamycine, sisomycine, neamine, neomycine-B and neomycine-C.
1-Fluoro-2,4-dinitrobenzene can also be used:
  • To examine the kinetic analysis and analytical uses of reaction with thiols in the presence of several surfactants.

Pictograms

Health hazardExclamation mark

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

327.2 °F

Flash Point(C)

164 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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F Wang et al.
Journal of chromatography. A, 883(1-2), 113-118 (2000-07-26)
A rapid and simple method is presented for determining neuro-excitatory nonprotein amino acid 3-N-oxalyl-2,3-diaminopropionic acid (beta-ODAP) and non-protein amino acids in Lathyrus sativus. Seed and foliage extracts of Lathyrus sativus were treated with 1-fluoro-2,4-dinitrobenzene (FDNB) and a reversed-phase high-performance liquid
G Paraskevas et al.
Journal of pharmaceutical and biomedical analysis, 29(5), 865-872 (2002-07-03)
A spectrophotometric method for the determination of lisinopril (LN) in single and multicomponent tablets also containing hydrochlorothiazide (HCT), based on the derivatization reaction with 1-fluoro-2,4-dinitrobenzene (FDNB, Sanger reagent) is described. Aqueous solutions of LN (4.5-27.2 x 10(-5) M) react with
Helena Shifrin et al.
PloS one, 8(2), e57668-e57668 (2013-03-08)
The cholinergic anti-inflammatory system and α7 nicotinic receptors in macrophages have been proposed to play a role in neuroimmunomodulation and in the etiology of ulcerative colitis. We investigated the ability of a cholinesterase (ChE) inhibitor rivastigmine, to improve the pathology
Ming-Feng Chiang et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(16), 6476-6481 (2013-04-12)
B lymphocyte-induced maturation protein-1 (Blimp-1) is a transcriptional repressor important for the differentiation and function of several types of immune cells. Because skin serves as a physical barrier and acts as an immune sentinel, we investigated whether Blimp-1 is involved
Sonia Schuepbach-Mallepell et al.
The Journal of allergy and clinical immunology, 132(6), 1348-1357 (2013-08-21)
Innate immune sensors control key cytokines that regulate T-cell priming and T-cell fate. This is particularly evident in allergic reactions, which represent ideal systems to study the interplay of innate and adaptive immunity. In patients with contact dermatitis, inflammasome-mediated IL-1

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    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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