252859

Tris Base

99.8-100.1%,≥99.8%, crystalline, ACS reagent

• Synonym(s): Tris(hydroxymethyl)aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol
• Linear Formula: NH₂C(CH₂OH)₃
• CAS Number: 77-86-1
• Molecular Weight: 121.14
• Beilstein: 741883
• EC Number: 201-064-4
• MDL number: MFCD00004679
• UNSPSC Code: 12352104
• PubChem Substance ID: 24855244
• NACRES: NA.25

Properties

• Product Name: Tris(hydroxymethyl)aminomethane, ACS reagent, ≥99.8%
• grade: ACS reagent
• Quality Level: 200
• Assay: ≥99.8%; 99.8-100.1%
• form: crystalline
• technique(s): affinity chromatography: suitable
• impurities: ≤0.005% insolubles; ≤2% water
• color: white
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• cation traces: Fe: ≤5 ppm; heavy metals: ≤5 ppm (ICP)
• absorption: passes test
• application(s): clinical research; diagnostic assay manufacturing; microbiology
• foreign activity: DNase, none detected; NICKase, none detected; RNase, none detected; protease, none detected
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma base, plays a crucial role in diverse research applications as a biological buffer. It is extensively used in the formulation of buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), owing to its optimal pKa of 8.1, making it well-suited for maintaining pH within the physiological range (pH 7 -9) applicable to most living organisms. However, caution might be necessary when studying some proteins, as Tris has the potential to interfere with the activity of certain enzymes. Tris base can be employed both independently as a buffer and as a component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, and TBE buffer, among others. Importantly, Tris base is characterized by its purity, essential stability, and relative non-hygroscopic nature.

In laboratory settings, Tris base is indispensable for the preparation of buffers compatible with biological fluids, acting as a standard pH solution. It facilitates various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it contributes to studies involving cell membrane permeability and buffer preparation.

Application

Tris(hydroxymethyl)aminomethane has been used:

• as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.[1][2][3]
• as a buffer in flow cell sample preparation for Raman spectroscopy[4]
• as a wash buffer in Western blotting[5]
• as a buffer component in decellularization of tissues[6]

Features and Benefits

• Efficient buffering within the pH range of 7-9 with a pKa of 8.1 (25 °C)
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Can be used in Cell Biology, and Biochemical research

Other Notes

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Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves